Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors
- Title
- Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors
- Author
- 오요한
- Keywords
- MYOBLAST FUSION; EXPRESSION; DIFFERENTIATION; MOUSE; GENES; SPECIFICATION; GENERATION; DERIVATION; DYSTROPHIN; EMBRYO
- Issue Date
- 2020-02
- Publisher
- ELIFE SCIENCES PUBLICATIONS LTD
- Citation
- ELIFE, v. 9, article no. e46981
- Abstract
- Generation of skeletal muscle cells with human pluripotent stem cells (hPSCs) opens new avenues for deciphering essential, but poorly understood aspects of transcriptional regulation in human myogenic specification. In this study, we characterized the transcriptional landscape of distinct human myogenic stages, including OCT4::EGFP+ pluripotent stem cells, MSGN1::EGFP+ presomite cells, PAX7::EGFP+ skeletal muscle progenitor cells, MYOG::EGFP+ myoblasts, and multinucleated myotubes. We defined signature gene expression profiles from each isolated cell population with unbiased clustering analysis, which provided unique insights into the transcriptional dynamics of human myogenesis from undifferentiated hPSCs to fully differentiated myotubes. Using a knock-out strategy, we identified TWIST1 as a critical factor in maintenance of human PAX7::EGFP+ putative skeletal muscle progenitor cells. Our data revealed a new role of TWIST1 in human skeletal muscle progenitors, and we have established a foundation to identify transcriptional regulations of human myogenic ontogeny.
- URI
- https://elifesciences.org/articles/46981https://repository.hanyang.ac.kr/handle/20.500.11754/161510
- ISSN
- 2050-084X
- DOI
- 10.7554/eLife.46981
- Appears in Collections:
- GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > BIOMEDICAL SCIENCE(의생명과학과) > Articles
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