Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오요한 | - |
dc.date.accessioned | 2021-04-16T00:30:58Z | - |
dc.date.available | 2021-04-16T00:30:58Z | - |
dc.date.issued | 2020-02 | - |
dc.identifier.citation | ELIFE, v. 9, article no. e46981 | en_US |
dc.identifier.issn | 2050-084X | - |
dc.identifier.uri | https://elifesciences.org/articles/46981 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/161510 | - |
dc.description.abstract | Generation of skeletal muscle cells with human pluripotent stem cells (hPSCs) opens new avenues for deciphering essential, but poorly understood aspects of transcriptional regulation in human myogenic specification. In this study, we characterized the transcriptional landscape of distinct human myogenic stages, including OCT4::EGFP+ pluripotent stem cells, MSGN1::EGFP+ presomite cells, PAX7::EGFP+ skeletal muscle progenitor cells, MYOG::EGFP+ myoblasts, and multinucleated myotubes. We defined signature gene expression profiles from each isolated cell population with unbiased clustering analysis, which provided unique insights into the transcriptional dynamics of human myogenesis from undifferentiated hPSCs to fully differentiated myotubes. Using a knock-out strategy, we identified TWIST1 as a critical factor in maintenance of human PAX7::EGFP+ putative skeletal muscle progenitor cells. Our data revealed a new role of TWIST1 in human skeletal muscle progenitors, and we have established a foundation to identify transcriptional regulations of human myogenic ontogeny. | en_US |
dc.description.sponsorship | National Institute of Arthritis and Musculoskeletal and Skin Diseases R01AR070751 Gabsang Lee Maryland Stem Cell Research Fund 2017-MSCRFD-3941 Gabsang Lee National Institutes of Health R01NS093213 Gabsang Lee Maryland Stem Cell Research Fund 2017-MSCRFD-3941 Gabsang Lee Muscular Dystrophy Association 381465 Gabsang Lee FSH Society FSHS-82014-03 Gabsang Lee National Research Foundation of Korea 2017K1A4A3014959 SangHwan Hyun Gabsang Lee The funders (NIAMS and MSCRF) had no role in study design, data collection and interpretation, or the decision to submit the work for publication. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELIFE SCIENCES PUBLICATIONS LTD | en_US |
dc.subject | MYOBLAST FUSION | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | DIFFERENTIATION | en_US |
dc.subject | MOUSE | en_US |
dc.subject | GENES | en_US |
dc.subject | SPECIFICATION | en_US |
dc.subject | GENERATION | en_US |
dc.subject | DERIVATION | en_US |
dc.subject | DYSTROPHIN | en_US |
dc.subject | EMBRYO | en_US |
dc.title | Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors | en_US |
dc.type | Article | en_US |
dc.relation.no | e46981 | - |
dc.relation.volume | 9 | - |
dc.identifier.doi | 10.7554/eLife.46981 | - |
dc.relation.page | 1-27 | - |
dc.relation.journal | ELIFE | - |
dc.contributor.googleauthor | Choi, In Young | - |
dc.contributor.googleauthor | Lim, Hotae | - |
dc.contributor.googleauthor | Cho, Hyeon Jin | - |
dc.contributor.googleauthor | Oh, Yohan | - |
dc.contributor.googleauthor | Chou, Bin-Kuan | - |
dc.contributor.googleauthor | Bai, Hao | - |
dc.contributor.googleauthor | Cheng, Linzhao | - |
dc.contributor.googleauthor | Kim, Yong Jun | - |
dc.contributor.googleauthor | Hyun, SangHwan | - |
dc.contributor.googleauthor | Kim, Hyesoo | - |
dc.relation.code | 2020046099 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOMEDICAL SCIENCE | - |
dc.identifier.pid | yoh | - |
dc.identifier.researcherID | C-9107-2013 | - |
dc.identifier.orcid | http://orcid.org/0000-0002-9249-8664 | - |
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