Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2021-03-29T02:15:32Z | - |
dc.date.available | 2021-03-29T02:15:32Z | - |
dc.date.issued | 2020-01 | - |
dc.identifier.citation | ENVIRONMENTAL SCIENCE & TECHNOLOGY, v. 54, no. 2, page. 1223-1231 | en_US |
dc.identifier.issn | 0013-936X | - |
dc.identifier.issn | 1520-5851 | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acs.est.9b05284 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/160912 | - |
dc.description.abstract | Carbonic anhydrases convert CO2 to bicarbonate at a high turnover rate up to 10(6) s(-1), but their actual applications in CO2 conversion processes are hampered by their poor stability. This study reports highly loaded and stabilized bovine carbonic anhydrase (bCA) upon being immobilized onto electrospun polymer nanofibers in the form of enzyme precipitate coating (EPC). The EPC protocol, consisting of enzyme covalent attachment, precipitation, and cross-linking, maintained 65.3% of initial activity even after being incubated in aqueous solution at room temperature under shaking at 200 rpm for 868 days. EPC also showed strong resistance to the treatment of the metal chelation agent, ethylenediaminetetraacetic acid, and molecular dynamic simulation was carried out to elucidate the prevention of metal leaching from the active site of bCA upon being cross-linked in the form of EPC. Highly stable EPC with high bCA loading was employed for the conversion of bubbling CO2 to bicarbonate, and the bicarbonate solution was utilized as a carbon source for expedited microalgae growth in a separate bioreactor. The addition of EPC in the bubbling CO2 reactor resulted in 134 and 231% accelerated microalgae growths compared to the controls with and without 25 mM sodium bicarbonate, respectively. EPC with high enzyme loading and unprecedentedly successful stabilization of enzyme stability has a great potential to be used for the development of various enzyme-mediated CO2 conversion and utilization technologies. | en_US |
dc.description.sponsorship | This work was supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (20118510020020, 20142020200980, and 20182010600430). This work was also supported by the "Global Research Laboratory Program" (2014K1A1A2043032) and "the Basic Core Technology Development Program for the Oceans and the Polar Regions" (NRF-201SM1A5A1037053) of the National Research Foundation of Korea (NRF) Grants, funded by the Korea government Ministry of Science and ICT. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | DIOXIDE | en_US |
dc.subject | CAPTURE | en_US |
dc.subject | BIOMASS | en_US |
dc.subject | NANOBIOCATALYSIS | en_US |
dc.subject | OPPORTUNITIES | en_US |
dc.subject | MECHANISMS | en_US |
dc.subject | DIGESTION | en_US |
dc.subject | PRODUCTS | en_US |
dc.subject | BIOFUELS | en_US |
dc.subject | COATINGS | en_US |
dc.title | Stabilized and Immobilized Carbonic Anhydrase on Electrospun Nanofibers for Enzymatic CO2 Conversion and Utilization in Expedited Microalgal Growth | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1021/acs.est.9b05284 | - |
dc.relation.page | 1223-1231 | - |
dc.relation.journal | ENVIRONMENTAL SCIENCE & TECHNOLOGY | - |
dc.contributor.googleauthor | Jun, Seung-Hyun | - |
dc.contributor.googleauthor | Yang, Jusang | - |
dc.contributor.googleauthor | Jeon, Hancheol | - |
dc.contributor.googleauthor | Kim, Han Sol | - |
dc.contributor.googleauthor | Pack, Seung Pil | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.contributor.googleauthor | Kim, Jungbae | - |
dc.relation.code | 2020046167 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
dc.identifier.researcherID | AAW-6839-2020 | - |
dc.identifier.orcid | http://orcid.org/0000-0001-5691-0124 | - |
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