Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이철훈 | - |
dc.date.accessioned | 2021-01-14T04:25:18Z | - |
dc.date.available | 2021-01-14T04:25:18Z | - |
dc.date.issued | 2002-06 | - |
dc.identifier.citation | Journal of Microbiology and Biotechnology, v. 12, no. 3, page. 470-475 | en_US |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | http://kiss.kstudy.com/thesis/thesis-view.asp?key=2752448 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/156976 | - |
dc.description.abstract | In the course of screening for a novel cell cycle inhibitor, a potent Cdk 1 inhibitor, HY558, was found from the culture broth of Penicillium wminioliteum F558 isolated from a soil sample. The molecular ion of HY558 was identified at m/z 329 (MH+) with a molecular formula of C20H44ON2. HY558 exhibited selective antiproliferative effects on various human cancer cell lines. Its IC50 values were estimated to be 0.29 mM on HepG2, 0.30 mM on HeLa, 0.30 mM on HL60, 0.33 mM on HT-29, and 0.25 mM on AGS cells. Interestingly, HY558 demonstrated no antiproliferative effect with normal lymphocytes used as the control, and a low level of inhibition on the proliferation of A549 cancer cells. A flow cytometric analysis of HepG2 cells revealed an appreciable arrest of cells at the G1 and G2/M phases of the cell cycle following treatment with HY558. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with 0.46 mM of HY558. | en_US |
dc.description.sponsorship | This work was supported by grant No. R01-2000-000-00106-0 from the Basic Research Program of the Korea Science & Engineering Foundation. All NMR measurements by the Bruker Avance 400 spectrometer system were partially supported by Shared Research Equipment Assistance Program 2001 of the Korea Basic Science Institute. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | 한국미생물생명공학회(구 한국산업미생물학회) | en_US |
dc.subject | HY558 | en_US |
dc.subject | cell cycle inhibitor | en_US |
dc.subject | Penicillium minioluteum F558 | en_US |
dc.title | Isolation and biological properties of novel cell cycle inhibitor, HY558, isolated from Penicillium minioluteum F558 | en_US |
dc.type | Article | en_US |
dc.relation.journal | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.googleauthor | Lee, Chul Hoon | - |
dc.contributor.googleauthor | Lim, Hae Young | - |
dc.contributor.googleauthor | Kim, Min Kyoung | - |
dc.contributor.googleauthor | Cho, Youl Hee | - |
dc.contributor.googleauthor | Oh, Deok Kun | - |
dc.contributor.googleauthor | Kim, Chang Jin | - |
dc.contributor.googleauthor | Lim, Yoong Ho | - |
dc.relation.code | 2008205425 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chhlee | - |
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