Identification of novel anti-angiogenic factors by in silico functional gene screening method
- Title
- Identification of novel anti-angiogenic factors by in silico functional gene screening method
- Author
- 마상백
- Keywords
- Angiogenesis; Transcription factor; Chromosomal localization; Gene screening
- Issue Date
- 2003-10
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- JOURNAL OF BIOTECHNOLOGY, v. 105, issue. 1-2, page. 51-60
- Abstract
- Angiogenesis, the formation of new blood vessels out of pre-existing capillaries, occurs in a variety of
pathophysiological conditions, and is regulated by a balance of angiogenic activators and inhibitors. To identify novel
angiogenic factors, we developed a gene screening method by combining the prediction analysis of transcription factor
(TF) binding site and the chromosomal localization analysis. First, we analyzed the promoter sequences from known
angiogenesis-related factors using the MATINSPECTOR program in TRANSFAC database. Interestingly, we found that
the binding site of LMO2 complex is highly conserved in the promoter regions of these factors. Second, we analyzed
chromosome loci based on the hypothesis that angiogenesis-related factors might be co-localized in a specific
chromosomal band. We found that angiogenesis-related factors are localized in specific 14 chromosomal bands
including 5q31 and 19q13 using AngioDB and LocusLink database mining. From these two approaches, we identified
32 novel candidates that have the LMO2 complex binding site in their promoter and are located on one of 14
chromosomal bands. Among them, human recombinant troponin T and spectrin markedly inhibited the neovascular-ization in vivo and in vitro. Collectively, we suggest that the combination of the prediction analysis of TF binding site
and the chromosomal localization analysis might be a useful strategy for gene screening of angiogenesis.
- URI
- https://www.sciencedirect.com/science/article/pii/S0168165603001834https://repository.hanyang.ac.kr/handle/20.500.11754/156388
- ISSN
- 0168-1656
- DOI
- 10.1016/S0168-1656(03)00183-4
- Appears in Collections:
- ETC[S] > 연구정보
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML