Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이철훈 | - |
dc.date.accessioned | 2020-12-11T04:56:44Z | - |
dc.date.available | 2020-12-11T04:56:44Z | - |
dc.date.issued | 2003-08 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v. 13, issue. 4, page. 607-612 | en_US |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | http://www.jmb.or.kr/journal/view.html?uid=1297&vmd=Full | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/156140 | - |
dc.description.abstract | To elucidate the action mechanism of MCS-C2, a novel analogue of toyocamycin and sangivamycin, its effect on the expression of cell cycle-related proteins in the human myelocytic leukemia cell line HL-60 was examined using Western blotting and a flow cytometric analysis. MCS-C2, a selective inhibitor of cyclin-dependent kinases, was found to inhibit cell growth in a time- and dose-dependent manner, and inhibits cell cycle progression by inducing the arrest at G1 and G2/M phases, in HL-60 cells. The flow cytometric analysis revealed an appreciable arrest of cells in the G2/M phase of the cell cycle after treatment with MCS-C2. The HL-60 cell population increased gradually from 13% at 0 h, to 28% at 12 h in the G2/M phase, after exposure to 2 muM MCS-C2. Furthermore, Western blot analysis demonstrated that MCS-C2 induced the cell cycle arrest at G1 phase through the inhibition of pRb phosphorylation. Hypophosphorylated pRb accumulated after treatment with 5 muM MCS-C2 for 12 h, whereas. the level of hyperphosphorylated pRb was reduced. Thus. treatment of the cell with MCS-C2 Suppressed the hyperphosphorylated form of pRb with a commensurate increase in the hypophosphorylated form. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | KOREAN SOC APPLIED MICROBIOLOGY(한국미생물.생명공학회) | en_US |
dc.subject | MCS-C2 | en_US |
dc.subject | toyocamycin | en_US |
dc.subject | cell-cycle arrest | en_US |
dc.subject | HL-60 | en_US |
dc.subject | cyclin-dependent kinase | en_US |
dc.title | Inhibition of cell-cycle progression in human promyelocytic leukemia HL-60 cells by MCS-C2, novel cyclin-dependent kinase inhibitor | en_US |
dc.type | Article | en_US |
dc.relation.journal | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.googleauthor | Kim, J.M. | - |
dc.contributor.googleauthor | Chun, M.W. | - |
dc.contributor.googleauthor | Lee, S.K. | - |
dc.contributor.googleauthor | Lim, Y. | - |
dc.contributor.googleauthor | Kim, M.K. | - |
dc.contributor.googleauthor | Cho, Y.-H. | - |
dc.contributor.googleauthor | Lee, C.-H. | - |
dc.relation.code | 2008205425 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chhlee | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.