Adenine base editors catalyze cytosine conversions in human cells
- Title
- Adenine base editors catalyze cytosine conversions in human cells
- Author
- 허준호
- Keywords
- GENOMIC DNA; TARGET
- Issue Date
- 2019-10
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- NATURE BIOTECHNOLOGY, v. 37, no. 10, Page. 1145-1148
- Abstract
- Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.
- URI
- https://www.nature.com/articles/s41587-019-0254-4https://repository.hanyang.ac.kr/handle/20.500.11754/154716
- ISSN
- 1087-0156; 1546-1696
- DOI
- 10.1038/s41587-019-0254-4
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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