Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김종만 | - |
dc.date.accessioned | 2020-10-20T00:14:14Z | - |
dc.date.available | 2020-10-20T00:14:14Z | - |
dc.date.issued | 2019-10 | - |
dc.identifier.citation | DYES AND PIGMENTS, v. 169, Page. 15-21 | en_US |
dc.identifier.issn | 0143-7208 | - |
dc.identifier.issn | 1873-3743 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0143720819305248?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/154652 | - |
dc.description.abstract | Urease is a virulence-factor enzyme found in various pathogenic bacteria. Many urinary-tract and gastro-intestinal diseases, including cancer, are related to urease activity, indicating that urease is critical to the human body. Herein, we report a colorimetric urease-sensing method using polydiacetylene vesicles. Due to the ammonia formed during urea hydrolyzation, the increase in local pH causes a color transition of the polydiacetylene vesicles from blue to red. Urease detection using this method is rapid, simple, sensitive, and economical. A minimum of 70 pU/ml of urease can be detected after 15 min of incubation, with a linearity range of 20 pU/ml-0.2 mU/ml. Urease detection in simulated gastric fluid was satisfactory, with a recovery percentage of 93.7-109.1%. The method was also used to screen the urease inhibitor acetohydroxamic acid, and the half-maximal inhibition value (IC50) was found to be 50 mM. | en_US |
dc.description.sponsorship | This investigation was supported financially by a grant from the National Research Foundation of Korea funded by the Korean government (MSIP) (NRF-2017R1A2A1A05000752). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Urease sensor | en_US |
dc.subject | Polydiacetylene vesicles | en_US |
dc.subject | pH-responsive sensor | en_US |
dc.title | pH-sensitive colorimetric polydiacetylene vesicles for urease sensing | en_US |
dc.type | Article | en_US |
dc.relation.volume | 169 | - |
dc.identifier.doi | 10.1016/j.dyepig.2019.04.072 | - |
dc.relation.page | 15-21 | - |
dc.relation.journal | DYES AND PIGMENTS | - |
dc.contributor.googleauthor | Jannah, Fadilatul | - |
dc.contributor.googleauthor | Kim, Jong-Man | - |
dc.relation.code | 2019002473 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF CHEMICAL ENGINEERING | - |
dc.identifier.pid | jmk | - |
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