Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 나승진 | - |
dc.date.accessioned | 2020-10-14T07:48:09Z | - |
dc.date.available | 2020-10-14T07:48:09Z | - |
dc.date.issued | 2019-10 | - |
dc.identifier.citation | JOURNAL OF PROTEOME RESEARCH, v. 18, no. 10, Page. 3800-3806 | en_US |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.issn | 1535-3907 | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00475 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/154582 | - |
dc.description.abstract | We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretomes. cRFP is a small supplementary sequence list of highly abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly. | en_US |
dc.description.sponsorship | The study was supported by a grant from the Korea Health Industry Development Institute (HI14C3484), grants from the National Research Foundation (2017M3A9F9030559, 2017M3C9A5031595, 2019M3E5D3073568), and an intramural program of the Korea Institute of Science and Technology. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | secretome | en_US |
dc.subject | common repository of FBS proteins | en_US |
dc.subject | database search | en_US |
dc.title | Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome | en_US |
dc.type | Article | en_US |
dc.relation.no | 10 | - |
dc.relation.volume | 18 | - |
dc.identifier.doi | 10.1021/acs.jproteome.9b00475 | - |
dc.relation.page | 3800-3806 | - |
dc.relation.journal | JOURNAL OF PROTEOME RESEARCH | - |
dc.contributor.googleauthor | Shin, Jihye | - |
dc.contributor.googleauthor | Kwon, Yumi | - |
dc.contributor.googleauthor | Lee, Seonjeong | - |
dc.contributor.googleauthor | Na, Seungjin | - |
dc.contributor.googleauthor | Hong, Eun Young | - |
dc.contributor.googleauthor | Ju, Shinyeong | - |
dc.contributor.googleauthor | Jung, Hyun-Gyo | - |
dc.contributor.googleauthor | Kaushal, Prashant | - |
dc.contributor.googleauthor | Shin, Sungho | - |
dc.contributor.googleauthor | Back, Ji Hyun | - |
dc.relation.code | 2019002332 | - |
dc.sector.campus | S | - |
dc.sector.daehak | RESEARCH INSTITUTE[S] | - |
dc.sector.department | INSTITUTION FOR ARTIFICIAL INTELLIGENCE RESEARCH HY_AIR | - |
dc.identifier.pid | sna | - |
dc.identifier.orcid | https://orcid.org/0000-0002-5159-2048 | - |
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