Effect of Relaxin Expression from an Alginate Gel-Encapsulated Adenovirus on Scar Remodeling in a Pig Model
- Title
- Effect of Relaxin Expression from an Alginate Gel-Encapsulated Adenovirus on Scar Remodeling in a Pig Model
- Author
- 윤채옥
- Keywords
- Gene therapy; scar remodeling; relaxin; adenovirus; alginate gel; pig scar model
- Issue Date
- 2019-09
- Publisher
- YONSEI UNIV COLL MEDICINE
- Citation
- YONSEI MEDICAL JOURNAL, v. 60, no. 9, Page. 854-863
- Abstract
- Purpose: Relaxin (RLX) is a transforming growth factor-beta 1 (TGF-beta 1) antagonist that is believed to function as a potent collagen re-arranger and a major suppressor of extracellular matrix components. Adenoviruses (Ads) are accepted vectors for cancer gene therapy. However, repeated treatments of Ad are limited by short-term biological activity in vivo. The efficacy of sustained RLX expression to scar remodeling was assessed using an injectable alginate gel-matrix system.
Materials and Methods: Pig scar tissue was treated with relaxin-expressing Ad loaded in alginate gel (gel/Ad-RLX). Surface areas, color, and pliability of scars were compared, and various factors influencing scar formation and collagen arrangement were analyzed.
Results: Gel/Ad-RLX decreased scar size, color index, and pliability. Immunohistochemistry showed decreased levels of major extracellular matrix proteins in the gel/Ad-RLX-treated group. Furthermore, treatment with gel/Ad-RLX reduced expression of tissue inhibitor of metalloproteinase-1 and alpha-smooth muscle actin and markedly increased expression of matrix metalloproteinase-1 in pig scar tissues. Gel/Ad-RLX also significantly downregulated TGF-beta 1 and upregulated TGF-beta 3 mRNAs in pig scar tissues.
Conclusion: These results support a prominent role for RLX in scar remodeling and suggest that gel/Ad-RLX may have therapeutic effects on scar formation.
- URI
- https://eymj.org/DOIx.php?id=10.3349/ymj.2019.60.9.854https://repository.hanyang.ac.kr/handle/20.500.11754/154071
- ISSN
- 0513-5796; 1976-2437
- DOI
- 10.3349/ymj.2019.60.9.854
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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- Effect of Relaxin Expression from an Alginate Gel-Encapsulated Adenovirus on Scar Remodeling in a Pig Model.pdfDownload
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