Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 송시몬 | - |
dc.date.accessioned | 2020-09-08T00:32:09Z | - |
dc.date.available | 2020-09-08T00:32:09Z | - |
dc.date.issued | 2019-08 | - |
dc.identifier.citation | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 41, article no. 101563 | en_US |
dc.identifier.issn | 2211-9264 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S2211926419301432?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/153634 | - |
dc.description.abstract | We quantitatively investigated Euglena gracilis photo-responses and photoadaptation under gravity by observing the distribution and movements of cells confined in a microchamber. All the movements in the microchamber were analyzed via video observation with longer/shorter time scales from seconds to hours. Normally the cells distributed at the upper area of the microchamber, exhibiting negative gravitaxis. Two types of negative gravitactic motions were suggested for non-explicit photo stimulation: circular swimming probably due to tail-heavy cell body and run and tumbling with a larger chance to turn upward. When the step-up or step-down photo-shock was induced by light stimulus, cells started sinking downward with on-site and continuous rotation. Positive phototaxis can be superimposed on negative gravitaxis, whereas negative phototaxis overcomes competing gravitaxis. The adaptation of cells to high-flux blue light (2780 mu mol/m(2) s, irradiated from the bottom) was clearly observed as cell distribution changed from the bottom (step-up photo-shock) to the top (negative phototaxis) of the microchamber. Faster adaptation was achieved by shortening the interval period of blue light stimulation. Our microchamber observation/analysis system is advantageous for quantitative investigation of photo-responses of microorganic cells under gravity, since the entire cellular movements in the microchamber can be tracked over time, from seconds to hours, with varying light illumination. | en_US |
dc.description.sponsorship | This work was supported by a grant for pioneering projects "Fundamental Principles Underlying the Hierarchy of Matter: A Comprehensive Experimental Study", provided by RIKEN. This research was also partially supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology [2016R1A2B3009541 and 2012R1A6A1029029]. A part of this work was supported by Japan Society for the Promotion of Science [grant number 17K07945] to Dr. T. Shinomura. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Phototaxis | en_US |
dc.subject | Photophobic responses | en_US |
dc.subject | Photo-shock | en_US |
dc.subject | Gravitaxis | en_US |
dc.subject | Blue-light | en_US |
dc.subject | Microfluidic devices | en_US |
dc.title | Phototaxis and photo-shock responses of Euglena gracilis under gravitaxis | en_US |
dc.type | Article | en_US |
dc.relation.volume | 41 | - |
dc.identifier.doi | 10.1016/j.algal.2019.101563 | - |
dc.relation.page | 1-11 | - |
dc.relation.journal | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | - |
dc.contributor.googleauthor | Ozasa, Kazunari | - |
dc.contributor.googleauthor | Won, June | - |
dc.contributor.googleauthor | Song, Simon | - |
dc.contributor.googleauthor | Shinomura, Tomoko | - |
dc.contributor.googleauthor | Maeda, Mizuo | - |
dc.relation.code | 2019036292 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DIVISION OF MECHANICAL ENGINEERING | - |
dc.identifier.pid | simonsong | - |
dc.identifier.researcherID | P-4656-2015 | - |
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