Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배상수 | - |
dc.date.accessioned | 2020-09-03T07:30:25Z | - |
dc.date.available | 2020-09-03T07:30:25Z | - |
dc.date.issued | 2019-08 | - |
dc.identifier.citation | MOLECULAR THERAPY, v. 27, no. 8, Page. 1364-1371 | en_US |
dc.identifier.issn | 1525-0016 | - |
dc.identifier.issn | 1525-0024 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1525001619302278?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/153541 | - |
dc.description.abstract | A nonsense mutation is a substitutive mutation in a DNA sequence that causes a premature termination during translation and produces stalled proteins, resulting in dysfunction of a gene. Although it usually induces severe genetic disorders, there are no definite methods for inducing read through of premature termination codons (PTCs). Here, we present a targeted tool for bypassing PTCs, named CRISPR-pass, that uses CRISPR-mediated adenine base editors. CRISPR-pass, which should be applicable to 95.5% of clinically significant nonsense mutations in the ClinVar database, rescues protein synthesis in patient-derived fibroblasts, suggesting potential clinical utility. | en_US |
dc.description.sponsorship | This work was supported by National Research Foundation of Korea (NRF) grants (2017R1A6A3A04004741 to D.H.J.; 2015M3A9E 6028949, 2017M3A9B4062654, and 2018M3D1A1058826 to Jeong H. Kim; and 2018M3A9H3022412 to S. B.); a grant from the Korea Research Institute of Standards and Science (KRISS - 2018 - GP2018-0018 to Jeong H. Kim); and grants from the Next Generation BioGreen 21 Program (PJ01319301), Korea Healthcare Technology R&D Project (HI16C1012), and Technology Innovation Program (20000158) (to S.B.). | en_US |
dc.language.iso | en | en_US |
dc.publisher | CELL PRESS | en_US |
dc.subject | GENOMIC DNA | en_US |
dc.subject | CELLS | en_US |
dc.title | CRISPR-Pass: Gene Rescue of Nonsense Mutations Using Adenine Base Editors | en_US |
dc.type | Article | en_US |
dc.relation.no | 8 | - |
dc.relation.volume | 27 | - |
dc.identifier.doi | 10.1016/j.ymthe.2019.05.013 | - |
dc.relation.page | 1364-1371 | - |
dc.relation.journal | MOLECULAR THERAPY | - |
dc.contributor.googleauthor | Lee, Choongil | - |
dc.contributor.googleauthor | Jo, Dong Hyun | - |
dc.contributor.googleauthor | Hwang, Gue-Ho | - |
dc.contributor.googleauthor | Yu, Jihyeon | - |
dc.contributor.googleauthor | Kim, Jin Hyoung | - |
dc.contributor.googleauthor | Park, Se-eun | - |
dc.contributor.googleauthor | Kim, Jin-Soo | - |
dc.contributor.googleauthor | Kim, Jeong Hun | - |
dc.contributor.googleauthor | Bae, Sangsu | - |
dc.relation.code | 2019002340 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF CHEMISTRY | - |
dc.identifier.pid | sangsubae | - |
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