Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최준호 | - |
dc.date.accessioned | 2020-06-03T05:31:40Z | - |
dc.date.available | 2020-06-03T05:31:40Z | - |
dc.date.issued | 2019-06 | - |
dc.identifier.citation | NATURE STRUCTURAL & MOLECULAR BIOLOGY, v. 26, no. 6, Page. 490-490 | en_US |
dc.identifier.issn | 1545-9993 | - |
dc.identifier.issn | 1545-9985 | - |
dc.identifier.uri | https://www.nature.com/articles/s41594-019-0234-x | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/151428 | - |
dc.description.abstract | Ribosomal RNA (rRNA) biogenesis is a multistep process requiring several nuclear and cytoplasmic exonucleases. The exact processing steps for mammalian 5.8S rRNA remain obscure. Here, using loss-of-function approaches in mouse embryonic stem cells (mESCs) and deep sequencing of rRNA intermediates, we investigate the requirements of exonucleases known to be involved in 5.8S maturation at nucleotide resolution and explore the role of the Perlman syndrome-associated 3'-5' exonuclease Dis3l2 in rRNA processing. We uncover a novel cytoplasmic intermediate that we name '7S(B)' rRNA that is generated through sequential processing by distinct exosome complexes. 7S(B) rRNA can be oligoadenylated by an unknown enzyme and/or oligouridylated by TUT4/7 and subsequently processed by Dis3l2 and Eri1. Moreover, exosome depletion triggers Dis3l2-mediated decay (DMD) as a surveillance pathway for rRNAs. Our data identify previously unknown 5.8S rRNA processing steps and provide nucleotide-level insight into the exonuclease requirements for mammalian rRNA processing. | en_US |
dc.description.sponsorship | This work was supported by grants to R.I.G. from the US National Institutes of Health (R01GM086386; R01CA211328) and the March of Dimes Foundation (FY15-3339). M.P. was supported by a research fellowship from the Manton Center for Orphan Disease Research. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | PERLMAN SYNDROME | en_US |
dc.subject | EXORIBONUCLEASE DIS3L2 | en_US |
dc.subject | PRERIBOSOMAL RNA | en_US |
dc.subject | QUALITY-CONTROL | en_US |
dc.subject | NONCODING RNAS | en_US |
dc.subject | CLEAVAGE SITES | en_US |
dc.subject | YEAST EXOSOME | en_US |
dc.subject | STEM-LOOP | en_US |
dc.subject | DEGRADATION | en_US |
dc.subject | PATHWAY | en_US |
dc.title | Exonuclease requirements for mammalian ribosomal RNA biogenesis and surveillance | en_US |
dc.type | Article | en_US |
dc.relation.no | 6 | - |
dc.relation.volume | 26 | - |
dc.identifier.doi | 10.1038/s41594-019-0234-x | - |
dc.relation.page | 490-490 | - |
dc.relation.journal | NATURE STRUCTURAL & MOLECULAR BIOLOGY | - |
dc.contributor.googleauthor | Pirouz, Mehdi | - |
dc.contributor.googleauthor | Munafo, Marzia | - |
dc.contributor.googleauthor | Ebrahimi, Aref G. | - |
dc.contributor.googleauthor | Choe, Junho | - |
dc.contributor.googleauthor | Gregory, Richard, I | - |
dc.relation.code | 2019001879 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | jcho2711 | - |
dc.identifier.orcid | https://orcid.org/0000-0002-2365-3755 | - |
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