Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이민형 | - |
dc.contributor.author | 현혜선 | - |
dc.date.accessioned | 2020-04-01T17:04:27Z | - |
dc.date.available | 2020-04-01T17:04:27Z | - |
dc.date.issued | 2010-02 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/142654 | - |
dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000413091 | en_US |
dc.description.abstract | Polyethylenimine (PEI) is well known for non-viral vector for the gene delivery. It has high transfection efficiency due to proton sponge effect. However, a commonly used PEI with a molecular weight of 25 kDa is highly toxic to cells, which limits its clinical applications. In this study, thus, non-toxic low molecular weight PEI (2 kDa) was conjugated with Dexamethasone as a gene carrier. Dexamethasone is an anti-inflammatory glucocorticoid. When dexamethasone binds to a glucocorticoid receptor after cellular entry, the receptor/dexamethasone complex is subsequently translocated into the nucleus. Therefore, if a gene carrier is conjugated with dexamethasone, the transport of DNA into the nucleus may be facilitated. Dexamethsone conjugated low molecular weight PEI 2kDa (PEI2K-Dexa) may have dual functions as an anti-inflammatory reagent and nuclear-targeting gene carrier. Heme oxygenase-1 (HO-1) is considered a protective gene in various diseases including stroke. The pSV-HO-1/polymer complexes were injected v directly cortex. Middle cerebral artery occlusion (MCAO) was performed at 1 hour after injection. Brain infarct size 24 hours after MCAO were evaluated by 2% 2, 3, 5-triphenyltetrazolium hydrochloride (TTC) staining. The cytokine levels of TNF-α and IL-1β were measured by ELISA. Also, ELISA for HO-1 was performed in infarcted area. The brain infarct size in the pEmpty/PEI2K-Dexa group was reduced significantly, compared with the pEmpty/PEI2K groups. The pSV-HO-1/PEI2K-Dexa group further reduced infarct size, compared with the pEmpty/PEI2K-Dexa group. The results suggest that PEI2K-Dexa has synergistic protective effect with HO-1 gene. Therefore, pSV-HO-1/PEI2K-Dexa complex is a potential candidate for therapeutic gene delivery to stroke. | - |
dc.publisher | 한양대학교 | - |
dc.title | Delivery of Heme Oxygenase-1 gene using Dexamethasone conjugated low molecular weight Polyethylenimine for stroke gene therapy | - |
dc.type | Theses | - |
dc.contributor.googleauthor | 현혜선 | - |
dc.sector.campus | S | - |
dc.sector.daehak | 대학원 | - |
dc.sector.department | 생명공학과 | - |
dc.description.degree | Master | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.