열대 해면 Xestospongia sp. 와 Agelas sp. 로부터 생리활성물질 추출연구

Abstract

해양천연물 학계의 세계적 석학인 페니컬교수는 “해양에는 지구상 생물의 80%가 존재하고 육상에 전혀 존재하지 않는 새로운 생물이 서식하고 있어 미래 신약개발의 보고가 될 것”이라고 전망했다. 실제로 해양에서는 16,000 종 이상의 2차 대사물질이 여러 종류의 해양생물로부터 얻어 졌으며 이들 중 많은 화합물들이 다양하고도 강력한 생리활성을 보여 주었다. 그 중에서 질소를 포함하는 알칼로이드 계열의 화합물은 세포독성, 항진균, 항말라리아 물질 등에 대해 강한 활성을 가지고 있고 의약품으로서 널리 이용 되고 있다. 따라서 본 연구에서는 이러한 화합물을 포함하는 새로운 생리활성 유용물질을 탐색하기 위해 마이크로네시아 연방국 축주에서 해면 Xestospongia sp. 을 선정하여 화학분석을 수행하였다. 이를 통해 15% aqueous MeOH추출물 에서 halenaquinone 유도체들을 분리하였고 이미 알려진 기지물질 7종과 함께 1종의 신물질을 발견하였다. 활성측정은 SRB assay결과 Adociaquinone B 화합물이 PC-3(전립선암)에 강한 활성을 보였다. 또한 해면 Agelas sp. 를 화학적으로 분석하여 bromopyrrole alkaloids 계열의 화합물중 4종의 기지물질과 1종의 신물질을 얻을 수 있었다. 신물질의 구조는 2D NMR과 HR/MS를 포함한 분광학적 방법들에 의해 확인하였다. 결과적으로 본 연구에서 수행되어 정제된 천연물은 향후 해양생명공학 연구의 중요한 소재로 사용될 수 있으며, 특히 천연물의 생리활성은 생화학 또는 의• 약학 분야 연구 및 신물질의 산업적 개발 활용 등에 중요한 정보가 될 것이다. |The world’s great schlolars on marine natural products in academia, according to professor Fenical “80% of marine organisms that exist on earth and on land that does not exist at all to form a new organism that’s going to be looking for the future drug development” he predicted. Consequently, more than 16,000 novel secondary metabolites have been isolated from various marine organisms and a great number of compounds have been reported to exhibit potent bioactivity.Among them, nitrogen-containg alkaloid compounds have shown to strong effect against cytotoxic, antifungal, antimalarial activity and they are widely used as medicine. In the course of our research for the bioactive secondary metabolites from marine organisms, we collected the marine sponge Xestospongia sp. from Chuuk, Federated States of Micronesia. The chemical investigation of the marine sponge Xestospongia sp. led to the isolation of new hexacyclic alkaloid compound together with previously reported seven compounds. For active measurements, SRB assay results Adociaquinone B compound showed a strong activity against PC-3 (prostate cancer).And studies on the chemical investigation of the Agelas sp. led to the isolation of a new bromopyrrole alkaloids compound together with previously reported four compounds. The structures of the novel compounds have been elucidated by combined spectroscopic methods including 2D NMR and HR/MS. This research has unambiguously demonstrated the biomedical potential of marine organisms. Compounds obtained during this research will be utilized as valuable assets for the development of medicines.; The world’s great schlolars on marine natural products in academia, according to professor Fenical “80% of marine organisms that exist on earth and on land that does not exist at all to form a new organism that’s going to be looking for the future drug development” he predicted. Consequently, more than 16,000 novel secondary metabolites have been isolated from various marine organisms and a great number of compounds have been reported to exhibit potent bioactivity.Among them, nitrogen-containg alkaloid compounds have shown to strong effect against cytotoxic, antifungal, antimalarial activity and they are widely used as medicine. In the course of our research for the bioactive secondary metabolites from marine organisms, we collected the marine sponge Xestospongia sp. from Chuuk, Federated States of Micronesia. The chemical investigation of the marine sponge Xestospongia sp. led to the isolation of new hexacyclic alkaloid compound together with previously reported seven compounds. For active measurements, SRB assay results Adociaquinone B compound showed a strong activity against PC-3 (prostate cancer).And studies on the chemical investigation of the Agelas sp. led to the isolation of a new bromopyrrole alkaloids compound together with previously reported four compounds. The structures of the novel compounds have been elucidated by combined spectroscopic methods including 2D NMR and HR/MS. This research has unambiguously demonstrated the biomedical potential of marine organisms. Compounds obtained during this research will be utilized as valuable assets for the development of medicines.