고분자와 생체물질의 잉크젯 프린팅 방법에 의한 바이오 센서와 세포 어레이 제작

Abstract

잉크젯 프린팅 기술은 잉크를 사용하여 이차원 패턴 혹은 삼차원 구조체를 만들어 내는 기술로써 일반 가정뿐아니라 산업 전분야에 걸쳐 활용되고 있는 기술이다. 특히 잉크젯 프린팅 방식 중 비접촉식 Drop-On-Demand 방식의 프린터가 1980년 개발되면서 응용분야를 산업분야로 점차 넓혀가고 있는 상황이다. 잉크젯 프린팅 기술은 생산공정을 줄일 수 있고, 청정공정(독성물질 사용 최소화)가 가능하며 기존의 공정과 비교하여 제어가 용이한 장점이 있다. 잉크젯 프린팅 기술을 사용하기 위해서는 잉크 특성 파악 및 토출되는 잉크 액적의 분사 조건을 맞춰야 하는 문제가 있다. 본 연구에서는 metal nano ink, conducting polymer ink, biocompatible polymer ink, biomacromolecule ink 등 다양한 재료를 이용하여 잉크젯 프린팅 기술 활용 가능성을 확인하였다. 또한, metal nano ink안정성을 높여주기 위한 분산제로 고분자계 계면활성제인 Pluronic F-127의 활용가능성을 확인하였으며, 이를 바탕으로 분산된 metal nano ink를 프린팅 하였다. 보다 우수한 프린팅 패턴을 얻기 위해 surface treatment방법을 이용하였으며, hydrophilic/hydrophobic 복합 처리 방법을 개발하였다. 그 외에도 conducting polymer, biocompatible polymer를 이용하여 프린팅 조건을 확인하였으며, biocompatible polymer를 프린팅하여 살아있는 세포를 패턴 위에서 배양 및 bioassay를 실시하였다. 세포 생활성에 영향을 주지 않으면서 세포를 원하는 패턴대로 만들어 배양이 가능하였으며, anticancer drug treatment 및 apoptosis분석 등 다양한 bioapplication이 가능함을 확인하였다. 최종적으로 bioelectronics 소자인 biosensor를 잉크젯 프린팅 방식으로 제작하여 그 성능을 확인하였다. Gas sensor 및 endotoxin sensor를 제작하였으며 전기화학적 검출 방식으로 sensor의 성능을 확인하였다. 높은 감도를 가지는 sensor를 잉크젯 프린팅 방식으로 제작이 가능함을 확인할 수 있었다. 잉크젯 프린팅 기술은 학술적인 분야뿐만 아니라 산업 분야에서 많은 활용이 가능할 것으로 기대된다. 또한, 연구 시제품 제작과 스케일업을 통한 생산에 활용이 가능할 것으로 기대된다.|Inkjet printers are widely used equipment in the home, office, and industrial fields. The inkjet printing technology is an interesting and versatile method to make 2-dimensional patterns or 3-dimensional structures from well defined ink solution with suitable geometric designs on various substrates. Non contact inkjet printing technology utilizing a Drop-On-Demand system was developed in the 1980s. After that inkjet printing technology has had a wide variety of applications. The inkjet printing technology has great advantages as compared with other process technologies. The most important advantage is simplicity, but the other advantages are environmental friendly and well controllable. In inkjet printing system, drops can be fired with consistency from a nozzle under pressure obtained from an electric pulse. Intensity and duration of inkjet printing conditions must be optimized for ink solutions to obtain stable drops in the volume range. Moreover, to produce fine lines or films, the ink frequency and relative velocity between print nozzle and substrate are set up to produce desired drop overlapping. In this study, we found inkjet printing feasibility using several kinds of inks which were metal nano ink, conducting polymer ink, biodegradable polymer ink, and biomacromolecule ink. Hydrophilic/hydrophobic complex plasma treatment as a progressive surface treatment method was developed to fabricate precise patterns. Different rates of O2/C4F8 plasma treatment allow the substrates, such as glass or film, to control surface energy. Metal nano inks were mixed with Pluronic F-127 which was a polymer surfactant for the dispersion of nano particles. Well dispersed metal nano inks were successfully printed by a piezoelectric inkjet printer. Biocompatible polymer inks also were fabricated and living cells were incubated on the fabricated pattern for arbitrary and complex cell patterning. The effects of a degree of apoptosis and cell proliferation were visualized on patterned cells on a glass substrate. Sensors were fabricated by using inkjet printer with metal nano ink and biomacromolecules on variable substrates. The performances of both were tested as bioelectronic devices. Efficiency tests of fabricated gas sensor and endotoxin sensor were conducted by electrochemical response detection. Finally, it has been confirmed that inkjet printers can fabricate various sensors having high selectivity and sensitivity. The overall results are expected to be values both as research application (making test devices), and in the industrial field (such as manufacturing).; Inkjet printers are widely used equipment in the home, office, and industrial fields. The inkjet printing technology is an interesting and versatile method to make 2-dimensional patterns or 3-dimensional structures from well defined ink solution with suitable geometric designs on various substrates. Non contact inkjet printing technology utilizing a Drop-On-Demand system was developed in the 1980s. After that inkjet printing technology has had a wide variety of applications. The inkjet printing technology has great advantages as compared with other process technologies. The most important advantage is simplicity, but the other advantages are environmental friendly and well controllable. In inkjet printing system, drops can be fired with consistency from a nozzle under pressure obtained from an electric pulse. Intensity and duration of inkjet printing conditions must be optimized for ink solutions to obtain stable drops in the volume range. Moreover, to produce fine lines or films, the ink frequency and relative velocity between print nozzle and substrate are set up to produce desired drop overlapping. In this study, we found inkjet printing feasibility using several kinds of inks which were metal nano ink, conducting polymer ink, biodegradable polymer ink, and biomacromolecule ink. Hydrophilic/hydrophobic complex plasma treatment as a progressive surface treatment method was developed to fabricate precise patterns. Different rates of O2/C4F8 plasma treatment allow the substrates, such as glass or film, to control surface energy. Metal nano inks were mixed with Pluronic F-127 which was a polymer surfactant for the dispersion of nano particles. Well dispersed metal nano inks were successfully printed by a piezoelectric inkjet printer. Biocompatible polymer inks also were fabricated and living cells were incubated on the fabricated pattern for arbitrary and complex cell patterning. The effects of a degree of apoptosis and cell proliferation were visualized on patterned cells on a glass substrate. Sensors were fabricated by using inkjet printer with metal nano ink and biomacromolecules on variable substrates. The performances of both were tested as bioelectronic devices. Efficiency tests of fabricated gas sensor and endotoxin sensor were conducted by electrochemical response detection. Finally, it has been confirmed that inkjet printers can fabricate various sensors having high selectivity and sensitivity. The overall results are expected to be values both as research application (making test devices), and in the industrial field (such as manufacturing).