마우스 모델에서 상처치유과정에 대한 지방줄기세포의 생물학적 영향

Abstract

Background: Adipose-tissue derived stem cells (ADSCs) are multi-potent cells that present a strong capacity to differentiate and proliferate to specific phenotypes. Although, ADSCs are known to facilitate wound healing of grafted skin lesions, their biologic effects related to tissue remodeling are not yet understood.

Objective: The aim of this study was to investigate the potential of ADSCs in enhancing wound healing, a possible up-regulation of cellular proliferation, migration, the expression of proliferating cell nuclear antigen(PCNA), and any possible activation of extracellular signal-regulated kinase(ERK) 1/2 pathway. Methods: Wound closure was measured in ADSC-, fibroblast-, and PBS-treated nude mice, and was plotted by time after wounding. Collagen deposition and angiogenesis were evaluated for histological study. In in vitro studies, collagen synthesis was assessed by RT-PCR, Western blotting, and immunofluorescent analysis.

Results: ADSC-implanted mice showed accelerated wound closure, with the closure rate increasing as early as on day 3, and also showed more new collagen deposition and angiogenesis than fibroblast-implanted mice. ADSCs also increased the ability of migration, perhaps, via activation of ERK1/2 signaling, and increased the proliferation, and for the expression of PCNA when compared to those of fibroblasts. ADSCs also showed higher levels of type I collagen expression than fibroblasts, as confirmed by RT-PCR, Western blot analysis, and immunofluorescence.

Conclusions: In this study, cell-implantation experiments showed that ADSCs can accelerate the wound healing process, increasing by not only migration and proliferation but also neocollagenosis and angiogenesis. This suggests that the ADSC could be an up-coming biologic tool for improving wound healing physiology.