Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이상경 | - |
dc.contributor.author | 김성화 | - |
dc.date.accessioned | 2020-02-27T16:32:24Z | - |
dc.date.available | 2020-02-27T16:32:24Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/131145 | - |
dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000423698 | en_US |
dc.description.abstract | Aggregation of β-amyloid peptides in the brain is neurotoxic and occurs in Alzheimer’s disease (AD). This peptide is generated from the trans-membrane domain of the Amyloid Precursor Protein (APP) and the gamma-secretase enzyme that is involved in the generation of this peptide is considered an important physiological target to inhibit β-amyloid accumulation. Direct inhibition of gamma-secretase using inhibitors is known to cause severe side effects as notch protein is one of the substrates of the gamma-secretase. shRNA knockdown of the gamma secretase activating protein (GASP) results in significant reduction in the generation of β-amyloid peptides in vitro and in vivo without effecting the notch signaling pathway. Here, we developed a novel system for brain delivery of siRNA, a peptide that targets the leptin receptor expressed on neurons (Lep) was conjugated with NONA-arginine (9R) that complexes siRNA. Lep-9R delivered functional siRNA to different regions of the mouse brain when administered systemically, particularly the hippocampus which is the major region of β-amyloid accumulation in AD. IV treatment with Lep-9R complexed siGSAP biweekly for 8 weeks inhibited β-amyloid accumulation by reducing brain levels of GSAP in the 3xTg-AD mouse model of AD. Further the treatment also ameliorated AD behavior in this mouse model. Our results indicate that inhibiting β-amyloid accumulation through knockdown of GSAP results in reducing AD progression and that Leptin-9R can be used for the delivery of therapeutic siRNA for CNS-related diseases. | - |
dc.publisher | 한양대학교 | - |
dc.title | 렙틴유래 펩티드를 이용한 siRNA의 뇌전달 연구 | - |
dc.title.alternative | Development and Investigation of a leptin-derived brain specific siRNA delivery system | - |
dc.type | Theses | - |
dc.contributor.googleauthor | 김성화 | - |
dc.sector.campus | S | - |
dc.sector.daehak | 대학원 | - |
dc.sector.department | 생명공학과 | - |
dc.description.degree | Master | - |
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