고지방식이를 섭취하는 쥐에서 과립구집락자극인자의 간지방증 예방 효과

Abstract

Background: Previously, the ameliorating effect of granulocyte-colony stimulating factor (G-CSF) in hepatic steatosis has been reported in experimental animals. It may also have preventive effects on the development of hepatic steatosis. Therefore, we investigated the preventive effects of G-CSF by using a high-fat diet (HFD) rat model.

Methods and Results: Fifteen rats were fed HFD and 9 rats were fed control diet from 10 weeks of age. Once mild steatosis was confirmed in the liver (after 10 weeks of feeding the HFD; at 20 weeks of age), HFD rats were randomly divided into two groups and treated with either G-CSF (100 µg kg-1 day-1 for 5 consecutive days every other week; HFD/G-CSF rats) or saline (HFD/saline rats) for 10 weeks at 20 weeks of age. All rats were sacrificed at 30 weeks of age. Histology was examined by hematoxylin and eosin (H-E) and Oil Red O staining, and the expression levels of genes of associated with lipogenesis and β-oxidation enzymes were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Histological examinations revealed that HFD/G-CSF rats had significantly lower lipid accumulation in their hepatocytes than did HFD/saline rats (p < 0.05). HFD/G-CSF rats also showed lower expression levels of genes associated with lipogenesis and higher expression levels of genes associated with β-oxidation than HFD/saline rats (p < 0.05).

Conclusion: In conclusion, we found that G-CSF prevented development of hepatic steatosis in a HFD rat model. The preventive effect may be associated with the regulation of gene expression involved in hepatic lipogenesis and β-oxidation.