Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김경수 | - |
dc.contributor.author | 주현우 | - |
dc.date.accessioned | 2020-02-19T16:30:45Z | - |
dc.date.available | 2020-02-19T16:30:45Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/127812 | - |
dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000427177 | en_US |
dc.description.abstract | Background: Previously, the ameliorating effect of granulocyte-colony stimulating factor (G-CSF) in hepatic steatosis has been reported in experimental animals. It may also have preventive effects on the development of hepatic steatosis. Therefore, we investigated the preventive effects of G-CSF by using a high-fat diet (HFD) rat model. Methods and Results: Fifteen rats were fed HFD and 9 rats were fed control diet from 10 weeks of age. Once mild steatosis was confirmed in the liver (after 10 weeks of feeding the HFD; at 20 weeks of age), HFD rats were randomly divided into two groups and treated with either G-CSF (100 µg kg-1 day-1 for 5 consecutive days every other week; HFD/G-CSF rats) or saline (HFD/saline rats) for 10 weeks at 20 weeks of age. All rats were sacrificed at 30 weeks of age. Histology was examined by hematoxylin and eosin (H-E) and Oil Red O staining, and the expression levels of genes of associated with lipogenesis and β-oxidation enzymes were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Histological examinations revealed that HFD/G-CSF rats had significantly lower lipid accumulation in their hepatocytes than did HFD/saline rats (p < 0.05). HFD/G-CSF rats also showed lower expression levels of genes associated with lipogenesis and higher expression levels of genes associated with β-oxidation than HFD/saline rats (p < 0.05). Conclusion: In conclusion, we found that G-CSF prevented development of hepatic steatosis in a HFD rat model. The preventive effect may be associated with the regulation of gene expression involved in hepatic lipogenesis and β-oxidation. | - |
dc.publisher | 한양대학교 | - |
dc.title | 고지방식이를 섭취하는 쥐에서 과립구집락자극인자의 간지방증 예방 효과 | - |
dc.title.alternative | Preventive effect of granulocyte-colony stimulating factor on the development of hepatic steatosis by high-fat diet in rats | - |
dc.type | Theses | - |
dc.contributor.googleauthor | 주현우 | - |
dc.contributor.alternativeauthor | Joo, Hyun Woo | - |
dc.sector.campus | S | - |
dc.sector.daehak | 의생명공학전문대학원 | - |
dc.sector.department | 임상의과학과 | - |
dc.description.degree | Master | - |
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