Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | MinHyung LEE | - |
dc.contributor.author | 담소남 | - |
dc.date.accessioned | 2020-02-18T16:35:56Z | - |
dc.date.available | 2020-02-18T16:35:56Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/127197 | - |
dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000427966 | en_US |
dc.description.abstract | Glioblastoma multiform (GBM) is one of the most common malignant primary brain tumors. It was previously reported that microRNA21 (miR-21), one of the glioma-specific microRNA, was an anti-apoptosis factor. miR-21 reduced the expression of tumor suppressor genes such as PDCD-4 and PTEN. Antagomir against miR-21 (AMO-21) may inhibit the action of miR-21 and reduce the growth of glioblastoma. In this study, deoxycholic acid conjugated polyethylenimine (DA3) was used as a carrier of miR-21. DA3 is an amphiphilic molecule with hydrophilic polyethylenimine and hydrophobic deoxycholic acid. Therefore, DA3 forms micelle in aqueous solution. Curcumin, which has anti-tumor effect, was loaded into the hydrophobic core of DA3 micelle, producing curcumin loaded DA3 (Curcumin-DA3). DA3-Curcumin formed stable complex with antagomir-21. In vitro transfection studies showed that DA3-Curcumin increased the delivery efficiency of antagomir-21 into the C6 rat glioblastoma cells, compared with polyethylenimine and Lipofectamine. Curcumin-DA3 increased the intracellular delivery of curcumin, compared with curcumin only or a simple mixture of DA3 and curcumin complex. As a result, Curcumin-DA3 showed higher anti-tumor effect than the controls. Furthermore, the delivery of antagomir-21(AMOs) with Curcumin-DA3 reduced viability of the C6 glioblastoma cells. Therefore, the complex of Curcumin-DA3 and antagomir-21 may be useful for the treatment of glioblastoma as a combination therapy of curcumin and antagomir-21. | - |
dc.publisher | 한양대학교 | - |
dc.title | Curcumin loaded DA3 as a carrier of the antagomir for the inhibition of microRNA-21 in glioblastoma | - |
dc.type | Theses | - |
dc.contributor.googleauthor | Xiaonan Tan | - |
dc.sector.campus | S | - |
dc.sector.daehak | 대학원 | - |
dc.sector.department | 생명공학과 | - |
dc.description.degree | Master | - |
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