암 줄기 세포 검출을 위한 생체적합성 저분자 펩타이드 개발 연구
- Title
- 암 줄기 세포 검출을 위한 생체적합성 저분자 펩타이드 개발 연구
- Other Titles
- Development of Small Peptides for the Cancer Stem Cell Detection
- Author
- 조준행
- Alternative Author(s)
- Cho, Jun Haeng
- Advisor(s)
- 윤문영
- Issue Date
- 2016-08
- Publisher
- 한양대학교
- Degree
- Doctor
- Abstract
- Cancer, also known as a malignant neoplasm, is a group of diseases in
which cells change and grow out of control. Technological advance and
better understanding of these cancer cell biology can effectively improve
the survival rate for nearly all types of cancer. However, the recurrence
and metastasis of cancer represent the elaborate obstacles to overcome in
the treatment of cancer. Recent studies have supported the cancer stem
cells (CSCs) hypothesis, which posits that tumors originate from tissue
stem cells or their early progenitors and as a result, produce tumors that
retain stem cell properties.
CSCs, as a subpopulation of cancer cells, have similar characteristics
with human normal stem cell that have the ability for self-renewal,
differentiation into various types of cancer cells and extensive proliferation.
Although traditional cancer therapies kill and reduce rapidly dividing cancer
cells, they may spare CSCs that can then give rise to a new tumor due to
the lack of target specificity to elucidate the CSCs. Thus, the diagnosis
and determination of prognosis made by CSCs markers could be a key
strategy to eradicate CSCs and cure the cancer.
In this study, we have initially screened the novel diverse peptides
displayed on M13 bacteriophages that binds to CD133, CD44 and PKC δ as
CSCs markers with high affinity (picomolar range). Based on a screened
peptides, we designed the nanocomplexation and functional peptides to
enhance the targeting efficiency towards CSCs markers. The peptide based
imaging agent designed in this study exhibited the stimulus responsive
fluorescence imaging property wih high accuracy towards CD133 as glioma
stem cell marker. Cooperative binding effect of PDPP (Polyvalent Directed
Peptide Polymer) successfully enhances the detection sensitivity on CD44
as breast cancer stem cell marker. As an inhibitor, the peptide possesses
higher inhibition potency than chemical drug towards PKC δ as glioma
stem cell marker.
The current study highlights the promising role of small peptides as
effective tool for detection the CSCs via active targeting. It is further
suggested that the conjugation of identified peptides with the various
nanomaterials would enhance their physicochemical stability and thus lead
to the CSCs detection system not only accurate targeting the CSCs marker
but also cellular imaging property.
In brief, identification of small peptide might be a useful strategy in the
development of target specific disease diagnostic system.
- URI
- https://repository.hanyang.ac.kr/handle/20.500.11754/125692http://hanyang.dcollection.net/common/orgView/200000486934
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > CHEMISTRY(화학과) > Theses (Ph.D.)
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