Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신경훈 | - |
dc.date.accessioned | 2019-12-19T01:19:11Z | - |
dc.date.available | 2019-12-19T01:19:11Z | - |
dc.date.issued | 2019-06 | - |
dc.identifier.citation | ENVIRONMENTAL SCIENCE & TECHNOLOGY, v. 53, No. 13, Page. 7830-7839 | en_US |
dc.identifier.issn | 0013-936X | - |
dc.identifier.issn | 1520-5851 | - |
dc.identifier.uri | https://pubs.acs.org/doi/abs/10.1021/acs.est.9b01359 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/121330 | - |
dc.description.abstract | To examine how tributyltin (TBT), a model obesogen, affects the lipid metabolism in the marine rotifer Brachionus koreanus, we carried out life-cycle studies and determined the in vitro and in silico interactions of retinoid X receptor (RXR) with TBT, the transcriptional levels of RXR and lipid metabolic genes, and the fatty acid content. The lethal concentration 10% (LC10) was determined to be 5.12 mu g/L TBT, and negative effects on ecologically relevant end points (e.g., decreased lifespan and fecundity) were detected at 5 mu g/L TBT. On the basis of these findings, subsequent experiments were conducted below 1 ug/L TBT, which did not show any negative effects on ecologically relevant end points in B. koreanus. Nile red staining analysis showed that after exposure to 1 mu g/L TBT, B. koreanus stored neutral lipids and had significantly increased transcriptional levels of RXR and lipid metabolism-related genes compared to the control. However, the content of total fatty acids did not significantly change at any exposure level. In the single fatty acids profile, a significant increase in saturated fatty acids (SFAs) 14:0 and 20:0 was observed, but the contents of omega-3 and omega-6 fatty acids were significantly decreased. Also, a transactivation assay of TBT with RXR showed that TBT is an agonist of Bk-RXR with a similar fold-induction to the positive control. Taken together, these results demonstrate that TBT-modulated RXR signaling leads to increase in transcriptional levels of lipid metabolism-related genes and the synthesis of SFAs but decreases the content of polyunsaturated fatty acids (PUFAs). Our findings support a wider taxonomic scope of lipid perturbation due to xenobiotic exposure that occurs via NRs in aquatic animals. | en_US |
dc.description.sponsorship | This work was supported by a grant (a base study to understand and counteract marine ecosystem change in Korean waters: Development of risk assessment and management process of ship's biofouling debris discharged from in water cleaning, PE99713) of Korea Institute of Ocean and Science Technology. LFCC's research (project no. 031342) is cofinanced by COMPETE 2020, Portugal 2020 and the European Union through the ERDF. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.title | Tributyltin Affects Retinoid X Receptor-Mediated Lipid Metabolism in the Marine Rotifer Brachionus koreanus | en_US |
dc.type | Article | en_US |
dc.relation.no | 13 | - |
dc.relation.volume | 53 | - |
dc.identifier.doi | 10.1021/acs.est.9b01359 | - |
dc.relation.page | 7830-7839 | - |
dc.relation.journal | ENVIRONMENTAL SCIENCE & TECHNOLOGY | - |
dc.contributor.googleauthor | Lee, M.-C. | - |
dc.contributor.googleauthor | Park, J.C. | - |
dc.contributor.googleauthor | Yoon, D.-S. | - |
dc.contributor.googleauthor | Han, J. | - |
dc.contributor.googleauthor | Lee, J.-S. | - |
dc.contributor.googleauthor | Fonseca, E. | - |
dc.contributor.googleauthor | Santos, M.L. | - |
dc.contributor.googleauthor | Castro, L.F.C. | - |
dc.contributor.googleauthor | Choi, H. | - |
dc.contributor.googleauthor | Shin, K.-H. | - |
dc.contributor.googleauthor | Kim, M. | - |
dc.contributor.googleauthor | Jung, J.-H. | - |
dc.contributor.googleauthor | Cho, H.-S | - |
dc.relation.code | 2019001173 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MARINE SCIENCE AND CONVERGENCE ENGINEERING | - |
dc.identifier.pid | shinkh | - |
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