Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 송시몬 | - |
dc.date.accessioned | 2019-12-10T04:50:26Z | - |
dc.date.available | 2019-12-10T04:50:26Z | - |
dc.date.issued | 2018-11 | - |
dc.identifier.citation | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, v. 35, page. 98-105 | en_US |
dc.identifier.issn | 2211-9264 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S2211926418303709?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/120725 | - |
dc.description.abstract | In this study, we developed a microfluidic system to elucidate the behavioral response of Euglena gracilis cells to simultaneous competing optical and chemical stimuli. The system illuminated a nonuniform blue light of 0.5-18 mW/cm(2) on cells confined in a 2D microchamber to induce a photophobic response (step-up photoshock). After cells accumulated in areas of weak blue light, a CO2 gradient (0%-100%) was generated in the microchamber to induce counter chemotaxis (aerotaxis). E. gracilis cells showed negative chemotaxis for areas of higher CO2 concentrations rather than strong blue light, suggesting that CO2 chemotaxis is dominant over blue light photophobicity. We also examined phototaxis instead of photo-shock responses, using in-plane uniform blue light illumination in a counter direction to the CO2 gradient. The cells exhibited both CO2 chemotaxis and blue light phototaxis, and swam back and forth randomly. In both cases, some sensitive cells accumulated in niche areas, primarily with CO2 concentration < 25% and secondarily with a weaker blue light intensity (further away from the blue light source). The results indicate that competing chemotactic and photophobic or phototactic stimuli induced superimposed responses, although E. gracilis was more strongly affected by CO2 chemotaxis than by blue light. We propose that the two independent chemotaxis and photoresponse signal-transduction pathways are merged into one flagellar control mechanism. Moreover, a small proportion of cells were resistant to CO2 or blue light, showing the diversity in cell metabolic status. | en_US |
dc.description.sponsorship | This work was supported by JSPS KAKENHI Grant Number JP25280092. This research was also partially supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology [2016R1A2B3009541 and 2012R1A6A1029029]. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Microfluidic device | en_US |
dc.subject | Chemotaxis | en_US |
dc.subject | Aerotaxis | en_US |
dc.subject | Step-up phototaxis | en_US |
dc.subject | Step-up photo-shock | en_US |
dc.subject | Euglena gracilis | en_US |
dc.title | Behavior of Euglena gracilis under simultaneous competing optical and chemical stimuli | en_US |
dc.type | Article | en_US |
dc.relation.volume | 35 | - |
dc.identifier.doi | 10.1016/j.algal.2018.08.013 | - |
dc.relation.page | 98-105 | - |
dc.relation.journal | Algal Research | - |
dc.contributor.googleauthor | Ozasa, Kazunari | - |
dc.contributor.googleauthor | Won, June | - |
dc.contributor.googleauthor | Song, Simon | - |
dc.contributor.googleauthor | Maeda, Mizuo | - |
dc.relation.code | 2018021154 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DIVISION OF MECHANICAL ENGINEERING | - |
dc.identifier.pid | simonsong | - |
dc.identifier.orcid | https://orcid.org/0000-0002-4043-9443 | - |
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