Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2019-12-04T04:47:41Z | - |
dc.date.available | 2019-12-04T04:47:41Z | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v. 115, no. 3, page. 719-728 | en_US |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.issn | 1097-0290 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1002/bit.26499 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/117154 | - |
dc.description.abstract | Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this study, we selected the C. reinhardtii CC-4349 strain as the best candidate among seven laboratory strains tested for carotenoid production. A knock-out mutant of the zeaxanthin epoxidase gene induced by preassembled DNA-free CRISPR-Cas9 ribonucleoproteins in the CC-4349 strain had a significantly higher zeaxanthin content (56-fold) and productivity (47-fold) than the wild type without the reduction in lutein level. Furthermore, we produced eggs fortified with lutein (2-fold) and zeaxanthin (2.2-fold) by feeding hens a diet containing the mutant. Our results clearly demonstrate the possibility of cost-effective commercial use of microalgal mutants induced by DNA-free CRISPR-Cas9 ribonucleoproteins in algal biotechnology for the production of high-value products. | en_US |
dc.description.sponsorship | Ministry of Science, ICT & Future Planning, Korea, Grant number: Korea CCS R&D Center (Korea CCS 2020 Project) (KCRC-2014M1A8A1049273); Ministry of Oceans and Fisheries, Korea, Grant number: Marine Biotechnology Program (20090267); Plant Molecular Breeding Center, Korea, Grant number: Next Generation BioGreen 21 Program (PJ01119201) | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | WILEY | en_US |
dc.subject | algal biotechnology | en_US |
dc.subject | Chlamydomonas reinhardtii | en_US |
dc.subject | CRISPR-Cas9 | en_US |
dc.subject | lutein | en_US |
dc.subject | zeaxanthin | en_US |
dc.title | Photoautotrophic production of macular pigment in a Chlamydomonas reinhardtii strain generated by using DNA-free CRISPR-Cas9 RNP-mediated mutagenesis | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 115 | - |
dc.identifier.doi | 10.1002/bit.26499 | - |
dc.relation.page | 719-728 | - |
dc.relation.journal | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.contributor.googleauthor | Baek, Kwangryul | - |
dc.contributor.googleauthor | Yu, Jihyeon | - |
dc.contributor.googleauthor | Jeong, Jooyeon | - |
dc.contributor.googleauthor | Sim, Sang Jun | - |
dc.contributor.googleauthor | Bae, Sangsu | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.relation.code | 2018003330 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.