Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이수재 | - |
dc.date.accessioned | 2019-11-29T22:18:31Z | - |
dc.date.available | 2019-11-29T22:18:31Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.citation | MICROCHIMICA ACTA, v. 184, no. 8, page. 2611-2620 | en_US |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.issn | 1436-5073 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs00604-017-2240-1 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/115307 | - |
dc.description.abstract | The authors describe three fluorescein-conjugated peptides generated by cell-phage display for use as a diagnostic probes for fluorescent detection and imaging of Salmonella enteritidis and Salmonella typhimurium. The authors also designed a polyvalent-directed peptide polymer synthesized with poly-D-lysine and bifunctional succinimidyl 3-(2pyridyldithio) propionate with an affinity and sensitivity that is higher by more than an order of magnitude compared to single peptides due to multiple binding site interactions. In order to establish a diagnostic system for food poisoning, imaging analysis was performed using fluorescence microscopy. The limit of detection of the diagnostic system based on polyvalent directed peptide interaction is 10(2) colony-forming units per mL for Salmonella. | en_US |
dc.description.sponsorship | This work was supported by the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ907052) funded by the Rural Development Administration and a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 201500000001213). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | SPRINGER WIEN | en_US |
dc.subject | Food poisoning | en_US |
dc.subject | Cell-phage display | en_US |
dc.subject | Polyvalant directed peptide polymer | en_US |
dc.subject | Fluorescein isothiocyanate | en_US |
dc.subject | Succinimidyl 3-(2-pyridyldithio)propionate | en_US |
dc.subject | RT-PCR | en_US |
dc.title | Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer | en_US |
dc.type | Article | en_US |
dc.relation.no | 8 | - |
dc.relation.volume | 184 | - |
dc.identifier.doi | 10.1007/s00604-017-2240-1 | - |
dc.relation.page | 2611-2620 | - |
dc.relation.journal | MICROCHIMICA ACTA | - |
dc.contributor.googleauthor | Lee, Sang-Choon | - |
dc.contributor.googleauthor | Kim, Min-Seo | - |
dc.contributor.googleauthor | Yoo, Ki-Chun | - |
dc.contributor.googleauthor | Ha, Na-Reum | - |
dc.contributor.googleauthor | Moon, Ji-Young | - |
dc.contributor.googleauthor | Lee, Su-Jae | - |
dc.contributor.googleauthor | Yoon, Moon-Young | - |
dc.relation.code | 2017002083 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | sj0420 | - |
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