Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김영필 | - |
dc.date.accessioned | 2019-11-27T19:38:19Z | - |
dc.date.available | 2019-11-27T19:38:19Z | - |
dc.date.issued | 2017-07 | - |
dc.identifier.citation | SENSORS AND ACTUATORS B-CHEMICAL, v. 246, page. 271-277 | en_US |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0925400517302940?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/114953 | - |
dc.description.abstract | Despite the simplicity and usability of gold nanoparticle (AuNP)-based colorimetry in a large panel of bioassays, this technique still suffers from a poor detection limit. To overcome this issue, we report an improved AuNP-based colorimetric assay in combination with simple centrifugation and silver enhancement. As a proof-of-concept, two types of enzymes (i.e., protein phosphatase and protease) were constructed based on the self-assembly of thiol-stabilized AuNPs in the presence of peptides and metal ions. When the AuNP solutions were subjected to a short cycle of centrifugation and silver enhancement, the signal-to-background ratio (SBR) was distinctly improved by a factor of 5-10 in both enzyme activity assays, as compared to that of AuNP-based colorimetry. The detection limit achieved by using the silver enhancement was determined to be improved by a factor of 1.0-3.4. Furthermore, grayscale images of the silver enhancement allowed for a rapid andsimple enzyme assay that did not require measuring.the absorbance. Due to its ability to improve the detection senstivity in a facile way, we anticipate that this approach will be suitable for use in many AuNP colorimetric assays. (C) 2017 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation (NRF) grant funded by the Korean government (MSIP) (No. 2016R1A2B2011744). This work was also supported by Basic Science Research Program (No. 2012R1A6A1029029) through the NRF funded by the Ministry of Education, Korea. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE SA | en_US |
dc.subject | Gold nanoparticle | en_US |
dc.subject | Silver enhancement | en_US |
dc.subject | Signal amplification | en_US |
dc.subject | Self-assembly | en_US |
dc.subject | Enzyme activity | en_US |
dc.title | Graying the self-assembly of gold nanoparticles for improved enzyme activity assays | en_US |
dc.type | Article | en_US |
dc.relation.volume | 246 | - |
dc.identifier.doi | 10.1016/j.snb.2017.02.067 | - |
dc.relation.page | 271-277 | - |
dc.relation.journal | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.contributor.googleauthor | Kim, Gae Bails | - |
dc.contributor.googleauthor | Lee, Jin Oh | - |
dc.contributor.googleauthor | Kim, Young-Pil | - |
dc.relation.code | 2017001010 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | ypilkim | - |
dc.identifier.researcherID | A-5437-2009 | - |
dc.identifier.orcid | http://orcid.org/0000-0001-7234-1320 | - |
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