Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2019-11-26T04:12:47Z | - |
dc.date.available | 2019-11-26T04:12:47Z | - |
dc.date.issued | 2017-06 | - |
dc.identifier.citation | BIO-PROTOCOL, v. 7, no. 11, Article no. e2352 | en_US |
dc.identifier.issn | 2331-8325 | - |
dc.identifier.uri | https://bio-protocol.org/e2352 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/114514 | - |
dc.description.abstract | We successfully introduced targeted knock-out of gene of interest in Chlamydomonas reinhardtii by using DNA-free CRISPR. In this protocol, the detailed procedures of an entire workflow cover from the initial target selection of CRISPR to the mutant analysis using next generation sequencing (NGS) technology. Furthermore, we introduce a web-based set of tools, named CRISPR RGEN tools (http://www.rgenome.net/), which provides all required tools from CRISPR target design to NGS data analysis. | en_US |
dc.description.sponsorship | This protocol was originally published as part of Baek et al. (2016). The authors especially thank Dr. Duk Hyoung Kim for developing the method. This work was supported by grants from the Korea CCS R&D Center (KCRC) (NRF-2014M1A8A1049273) to E.J. and the Plant Molecular Breeding Center of Next Generation BioGreen 21 Program (PJ01119201) to S.B. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BIO-PROTOCOL | en_US |
dc.subject | Genome editing | en_US |
dc.subject | CRISPR-Cas9 | en_US |
dc.subject | Microalgae | en_US |
dc.subject | Ribonucleoproteins | en_US |
dc.subject | Chlamydomonas reinhardtii | en_US |
dc.subject | DNA-free transformation | en_US |
dc.title | DNA-free Genome Editing of Chlamydomonas reinhardtii Using CRISPR and Subsequent Mutant Analysis | en_US |
dc.type | Article | en_US |
dc.relation.no | 11 | - |
dc.relation.volume | 7 | - |
dc.identifier.doi | 10.21769/BioProtoc.2352 | - |
dc.relation.page | 1-14 | - |
dc.relation.journal | bio-protocol | - |
dc.contributor.googleauthor | Yu, Jihyeon | - |
dc.contributor.googleauthor | Baek, Kwangryul | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.contributor.googleauthor | Bae, Sangsu | - |
dc.relation.code | 2016042190 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
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