Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 노미나 | - |
dc.date.accessioned | 2019-11-25T04:22:04Z | - |
dc.date.available | 2019-11-25T04:22:04Z | - |
dc.date.issued | 2017-05 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE, v. 49, Article no. e330 | en_US |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://www.nature.com/articles/emm201747 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/114052 | - |
dc.description.abstract | Evidence indicates that Helicobacter pylori is the causative agent of chronic gastritis and perhaps gastric malignancy. Extracellular vesicles (EVs) play an important role in the evolutional process of malignancy due to their genetic material cargo. We aimed to evaluate the clinical significance and biological mechanism of H. pylori EVs on the pathogenesis of gastric malignancy. We performed 16S rDNA-based metagenomic analysis of gastric juices either from endoscopic or surgical patients. From each sample of gastric juices, the bacteria and EVs were isolated. We evaluated the role of H. pylori EVs on the development of gastric inflammation in vitro and in vivo. IVIS spectrum and confocal microscopy were used to examine the distribution of EVs. The metagenomic analyses of the bacteria and EVs showed that Helicobacter and Streptococcus are the two major bacterial genera, and they were significantly increased in abundance in gastric cancer (GC) patients. H. pylori EVs are spherical and contain CagA and VacA. They can induce the production of tumor necrosis factor-alpha, interleukin (IL)-6 and IL-1 beta by macrophages, and IL-8 by gastric epithelial cells. Also, EVs induce the expression of interferon gamma, IL-17 and EV-specific immunoglobulin Gs in vivo in mice. EVs were shown to infiltrate and remain in the mouse stomach for an extended time. H. pylori EVs, which are abundant in the gastric juices of GC patients, can induce inflammation and possibly cancer in the stomach, mainly via the production of inflammatory mediators from gastric epithelial cells after selective uptake by the cells. | en_US |
dc.description.sponsorship | We thank the animal facility members of Pohang University of Science and Technology Biotech Center (Pohang University of Science and Technology, Pohang, Republic of Korea). This work was funded by the Ministry of Science, ICT, and Future Planning (NRF-2014R1A1A1005144 and NRF-2014K1B1A1073720), by the Basic Science Research Program funded by the Ministry of Education (NRF-2012R1A1A2007865) and by Korea Ministry of Health & Welfare, Republic of Korea (HI16C0998). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | OUTER-MEMBRANE VESICLES | en_US |
dc.subject | NF-KAPPA-B | en_US |
dc.subject | VACUOLATING CYTOTOXIN | en_US |
dc.subject | CLINICAL-RELEVANCE | en_US |
dc.subject | IL-8 PRODUCTION | en_US |
dc.subject | MICROBIOTA | en_US |
dc.subject | SECRETION | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | EXOSOMES | en_US |
dc.subject | PROTEIN | en_US |
dc.title | Helicobacter pylori-derived extracellular vesicles increased in the gastric juices of gastric adenocarcinoma patients and induced inflammation mainly via specific targeting of gastric epithelial cells | en_US |
dc.type | Article | en_US |
dc.relation.volume | 49 | - |
dc.identifier.doi | 10.1038/emm.2017.47 | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.contributor.googleauthor | Choi, Hyun-Il | - |
dc.contributor.googleauthor | Choi, Jun-Pyo | - |
dc.contributor.googleauthor | Seo, Jiwon | - |
dc.contributor.googleauthor | Kim, Beom Jin | - |
dc.contributor.googleauthor | Rho, Mina | - |
dc.contributor.googleauthor | Han, Jin Kwan | - |
dc.contributor.googleauthor | Kim, Jae Gyu | - |
dc.relation.code | 2017002500 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF COMPUTER SCIENCE | - |
dc.identifier.pid | minarho | - |
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