Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 류종석 | - |
dc.date.accessioned | 2019-11-21T01:30:26Z | - |
dc.date.available | 2019-11-21T01:30:26Z | - |
dc.date.issued | 2019-04 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v. 512, No. 2, Page. 314-318 | en_US |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0006291X19304292 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/113017 | - |
dc.description.abstract | Plasmin is a potent serin protease involved in a variety of biological functions, such as fibrinolysis and tissue remodeling. On performing an in vitro control assay to measure the activity of endogenous plasmin in cell lysates, a stimulatory effect of non-ionic detergent NP-40 on plasmin activity was discovered. Another non-ionic detergent, TX-100, also enhanced plasmin activity, while ionic detergents sodium deoxycholate and sodiem dodecyl sulfate abolished plasmin enzyme activity. Kinetic analysis of plasmin activity in the presence of NP-40 and TX-100 demonstrated an increase in V-max; however, there was no change in K-m values, suggesting that these detergents stimulate plasmin activity in a non-competitive manner. Fibrin plate assay indicates that NP-40 and TX-100 functionally stimulate plasmin activity by showing a dose-dependent increase in fibrinolysis. (C) 2019 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the research fund of Hanyang University (HY-2014-P). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
dc.subject | Non-ionic | en_US |
dc.subject | Detergent | en_US |
dc.subject | Enzyme | en_US |
dc.subject | Plasmin | en_US |
dc.subject | Non-competitive | en_US |
dc.subject | Stimulator | en_US |
dc.title | Non-ionic detergents Nonidet P-40 and Triton X-100 increase enzymatic activity of plasmin | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 512 | - |
dc.identifier.doi | 10.1016/j.bbrc.2019.03.052 | - |
dc.relation.page | 314-318 | - |
dc.relation.journal | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.googleauthor | Trinh, Trang H. T. | - |
dc.contributor.googleauthor | Kim, Jeeyoung | - |
dc.contributor.googleauthor | Lee, Chul-Hoon | - |
dc.contributor.googleauthor | Ryou, Chongsuk | - |
dc.relation.code | 2019001376 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | cryou2 | - |
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