Nestin Expression in the Adult Mouse Retina with Pharmaceutically Induced Retinal Degeneration
- Title
- Nestin Expression in the Adult Mouse Retina with Pharmaceutically Induced Retinal Degeneration
- Author
- 조희윤
- Keywords
- Nestin; N-methyl-N-nitrosourea (MNU); Retinal Degeneration; Mouse
- Issue Date
- 2017-02
- Publisher
- KOREAN ACAD MEDICAL SCIENCES
- Citation
- JOURNAL OF KOREAN MEDICAL SCIENCE, v. 32, no. 2, page. 343-351
- Abstract
- The present study investigated the temporal pattern and cellular localization of nestin in the adult mouse retina with pharmaceutically induced retinal degeneration using N-methyl-N-nitrosourea (MNU). After a single intraperitoneal injection of MNU in 8-weekold C57BL/6 mice, the animals were sacrificed at 1, 3, 5, 7, and 21 days (n=6, in each stage). The eyes were examined by means of immunohistochemical tests using nestin, ionized calcium-binding adaptor molecule (Iba-1), CD11b, F4/80, and glial fibrillary acidic protein (GFAP). Western blot analysis and manual cell counting were performed for quantification. Nestin expression was increased after MNU administration. Nestin+/Iba-1+ cells were migrated into outer nuclear layer (ONL) and peaked at day 3 post injection (PI). Nestin+/CD11b+ cells were also mainly identified in ONL at day 3 PI and peaked at day 5. Nestin+/F4/80+ cells were shown in the subretinal space and peaked at day 3 PI. Nestin+/GFAP+ cells were distinctly increased at day 1 PI and peaked at day 5 PI. The up-regulation of nestin expression after MNU administration in adult mouse retinal microglia, and monocyte/macrophage suggests that when retinal degeneration progresses, these cells may revert to a more developmentally immature state. Muller cells also showed reactive gliosis and differentiational changes.
- URI
- https://jkms.org/DOIx.php?id=10.3346/jkms.2017.32.2.343https://repository.hanyang.ac.kr/handle/20.500.11754/112912
- ISSN
- 1011-8934; 1598-6357
- DOI
- 10.3346/jkms.2017.32.2.343
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
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