Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이철훈 | - |
dc.date.accessioned | 2019-10-07T02:34:41Z | - |
dc.date.available | 2019-10-07T02:34:41Z | - |
dc.date.issued | 2005-06 | - |
dc.identifier.citation | CANCER LETTERS, v. 223, No. 2, Page. 239-247 | en_US |
dc.identifier.issn | 0304-3835 | - |
dc.identifier.issn | 1872-7980 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0304383504008547 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/110887 | - |
dc.description.abstract | The purpose of the present study was to investigate the anti-proliferative and apoptotic effects of MCS-C2, a novel synthetic analogue of the pyrrolo[2,3-d]pyrimidine nucleoside toyocamycin and sangivamycin, in human promyelocytic leukemia (HL-60) cells. When treated with 5 mu M MCS-C2, inhibited proliferation associated with apoptotic induction was found in the HL-60 cells in a concentration-dependent and time-dependent manner, plus nuclear DAPI staining revealed the typical nuclear features of apoptosis. However, MCS-C2 showed almost no antiproliferative effect and no apoptotic induction in normal lymphocyte cells used as a control when compared with those in HL-60 cancer cells. Moreover, a flow cytometric analysis of the HL-60 cells using FITC-dUTP and propidium iodide (PI) showed that the apoptotic cell population increased gradually from < 1% at 0 h to 34% at 12 h after exposure to 5 mu M MCS-C2. This apoptotic induction was associated with the cleavage of Bid and a release of cytochrome c from mitochondria into the cytosol, followed by the activation of caspase-3 and inactivation of poly(ADP-ribose) polymerase (PARP). However, there was no significant change in any other mitochondrial membrane proteins, such as Bcl-2 and Bax. Consequently, the current findings suggest that the mitochondrial pathway was primarily involved in the MCS-C2-induced apoptosis in the human promyelocytic leukemia HL-60 cells. (c) 2004 Elsevier Ireland Ltd. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by Research Funds from Hanyang University (HY-2002-T) and by the R & D program for Fusion strategy of Advanced Technologies (Korea Ministry of Science and Technology). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCI IRELAND LTD | en_US |
dc.subject | MCS-C2 | en_US |
dc.subject | leukemia cell | en_US |
dc.subject | cytochrome c | en_US |
dc.subject | apoptosis | en_US |
dc.subject | bid | en_US |
dc.title | Induction of apoptosis in human leukemia cells by MCS-C2 via caspase-dependent Bid cleavage and cytochrome c relaese | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.canlet.2004.10.045 | - |
dc.relation.journal | CANCER LETTERS | - |
dc.contributor.googleauthor | Kim, MK | - |
dc.contributor.googleauthor | Cho, YH | - |
dc.contributor.googleauthor | Kim, JM | - |
dc.contributor.googleauthor | Chun, MW | - |
dc.contributor.googleauthor | Lee, SK | - |
dc.contributor.googleauthor | Lim, Y | - |
dc.contributor.googleauthor | Lee, CH | - |
dc.relation.code | 2009201684 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chhlee | - |
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