Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 안성훈 | - |
dc.date.accessioned | 2019-06-11T07:07:08Z | - |
dc.date.available | 2019-06-11T07:07:08Z | - |
dc.date.issued | 2007-04 | - |
dc.identifier.citation | JOURNAL OF PHARMACY AND PHARMACOLOGY, v. 59, No. 4, Page. 561-566 | en_US |
dc.identifier.issn | 0022-3573 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1211/jpp.59.4.0011 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/106432 | - |
dc.description.abstract | We have previously reported that ergolide, a sesquiterpene lactone isolated from Inula britannica, suppresses inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression by inhibiting nuclear factor-kappa B (NF-kappa B) in RAW 264.7 macrophages. In this study, we show that ergolide suppresses the DNA binding activity of NF-kappa B and nuclear translocation of NF-kappa B p65 subunit, leading to the inhibition of NF-kappa B-dependent gene transcription in 12-O-tetradecanoylphorbol 13-acetate (TPA)-stimulated HeLa cells. We also show that ergolide decreases the degradation and phosphorylation of I kappa B, an inhibitory protein of NF-kappa B, and this effect is accompanied by a simultaneous reduction of I kappa B kinase (IKK) activity. However, ergolide does not inhibit in-vitro IKK activity directly, suggesting the possible involvement of upstream IKK kinases in the regulation of NF-kappa B activation. Furthermore, ergolide-mediated protein kinase C alpha (PKC alpha) inhibition is involved in reduction of NF-kappa B inhibition, as demonstrated by the observation that dominant negative PKC alpha, but not p44/42 MAPK and p38 MAPK, inhibits TPA-stimulated reporter gene expression. Taken together, our results suggest that ergolide suppresses NF-kappa B activation through the inhibition of PKC alpha-IKK activity, providing insight for PKC alpha as a molecular target for anti-inflammatory drugs. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | PHARMACEUTICAL PRESS-ROYAL PHARMACEUTICAL SOC GREAT BRITIAN | en_US |
dc.subject | NITRIC-OXIDE SYNTHASE | en_US |
dc.subject | PROTEIN-KINASE-C | en_US |
dc.subject | DNA-BINDING ACTIVITY | en_US |
dc.subject | RAW-264.7 MACROPHAGES | en_US |
dc.subject | ACTIVATION | en_US |
dc.subject | INHIBITION | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | ALPHA | en_US |
dc.subject | BETA | en_US |
dc.subject | IKK | en_US |
dc.title | Suppression of the NF‐kB signalling pathway by ergolide, sesquiterpene lactone, in HeLa cells | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1211/jpp.59.4.0011 | - |
dc.relation.journal | JOURNAL OF PHARMACY AND PHARMACOLOGY | - |
dc.contributor.googleauthor | Chun, Jae Kwang | - |
dc.contributor.googleauthor | Seo, Dong-Wan | - |
dc.contributor.googleauthor | Ahn, Seong Hoon | - |
dc.contributor.googleauthor | Park, Jae Hyun | - |
dc.contributor.googleauthor | You, Jueng-Soo | - |
dc.contributor.googleauthor | Lee, Chang-Hee | - |
dc.contributor.googleauthor | Lee, Jae Cheol | - |
dc.contributor.googleauthor | Kim, Yong Kee | - |
dc.contributor.googleauthor | Han, Jeung-Whan | - |
dc.relation.code | 2007205630 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MOLECULAR AND LIFE SCIENCE | - |
dc.identifier.pid | hoon320 | - |
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