Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤준용 | - |
dc.date.accessioned | 2019-06-10T04:21:36Z | - |
dc.date.available | 2019-06-10T04:21:36Z | - |
dc.date.issued | 2007-03 | - |
dc.identifier.citation | JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v. 103, No. 3, Page. 242-246 | en_US |
dc.identifier.issn | 1389-1723 | - |
dc.identifier.issn | 1347-4421 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1389172307700543 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/106335 | - |
dc.description.abstract | Genomic DNA from solid biomaterials was directly isolated with an automated DNA extractor, which was based on magnetic bead technology with a bore-mediated grinding (BMG) system. The movement of the bore broke down the solid biomaterials, mixed crude lysates thoroughly with reagents to isolate the DNA, and carried the beads to the next step. The BMG system was suitable for the mechanical homogenization of the solid biomaterials and valid as an automated system for purifying the DNA from the solid biomaterials without the need for pretreatment or disruption procedures prior to the application of the solid biomaterials. | en_US |
dc.description.sponsorship | We would like to thank Dr. Juen-Ho Shin for general discussion and providing biological samples. We are very grateful to the anonymous reviewers for reading and critical comments. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | SOC BIOSCIENCE BIOENGINEERING JAPAN | en_US |
dc.subject | DNA isolation | en_US |
dc.subject | solid biological source | en_US |
dc.subject | magnetic bead | en_US |
dc.title | Direct DNA Isolation from Solid Biological Sources without Pretreatments with Proteinase-K and/or Homogenization through Automated DNA Extraction | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1263/jbb.103.242 | - |
dc.relation.journal | JOURNAL OF BIOSCIENCE AND BIOENGINEERING | - |
dc.contributor.googleauthor | Ki, Jang-Seu | - |
dc.contributor.googleauthor | Chang, Ki Byum | - |
dc.contributor.googleauthor | Roh, Hee June | - |
dc.contributor.googleauthor | Lee, Bong Youb | - |
dc.contributor.googleauthor | Yoon, Joon Yong | - |
dc.contributor.googleauthor | Jang, Gi Young | - |
dc.relation.code | 2007204750 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF MECHANICAL ENGINEERING | - |
dc.identifier.pid | joyoon | - |
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