Microfabricated multilayer parylene-C stencils for the generation of patterned dynamic co-cultures
- Title
- Microfabricated multilayer parylene-C stencils for the generation of patterned dynamic co-cultures
- Author
- 정봉근
- Keywords
- cellular microenvironment; dynamic co-culture; parylene-C stencils; cell patterning
- Issue Date
- 2008-07
- Publisher
- WILEY-LISS
- Citation
- JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, v. 86A, No. 1, Page. 278-288
- Abstract
- Co-culturing different cell types can be useful to engineer a more in vivo-like microenvironment for cells in culture. Recent approaches to generating cellular co-cultures have used microfabrication technologies to regulate the degree of cell-cell contact between different cell types. However, these approaches are often limited to the co-culture of only two cell types in static cultures. The dynamic aspect of cell-cell interaction, however, is a key regulator of many biological processes such as early development, stem cell differentiation, and tissue regeneration. In this study, we describe a micropatterning technique based on microfabricated multilayer parylene-C stencils and demonstrate the potential of parylene-C technology for co-patterning of proteins and cells with the ability to generate a series of at least five temporally controlled patterned cocultures. We generated dynamic co-cultures of murine embryonic stem cells in culture with various secondary cell types that could be sequentially introduced and removed from the co-cultures. Our studies suggested that dynamic co-cultures generated by using parylene-C stencils may be applicable in studies investigating cellular interactions in controlled microenvironments such as studies of ES cell differentiation, wound healing and development. (c) 2008 Wiley Periodicals, Inc.
- URI
- https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.32030https://repository.hanyang.ac.kr/handle/20.500.11754/104754
- ISSN
- 1549-3296
- DOI
- 10.1002/jbm.a.32030
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML