Peptide phage display 기술을 이용한 나노입자의 materials recognition 응용 - Part Ⅱ: Fe3O4 나노입자를 이용한 magnetic bio-panning

Abstract

The magnetism of Fe3O4 nanoparticles was applied to magnetic bio-panning process for finding specific sequences against Fe3O4 crystal phase. Vibrating sample magnetometer (VSM) measurement showed that the coercivity of 30 Oe and the saturation magnetization of 55 emu/g were sufficient in controlling particle movement and magnetizing particles in the media, respectively. This ferrimagnetism of nanoparticles practically enhanced panning efficiency by exaggerating centrifuge step and preventing particle loss. Sequencing results showed that histidine which was commonly found in peptide sequences played an important role in the binding onto Fe3O4 nanoparticle surface. However, various possible motifs were also observed from several neighboring amino acids of histidine.