Synthesis and optimization of ceftriaxone-loaded solid lipid nanocarriers

Abstract

The use of nanocarriers to enhance drug delivery efficacy has been increasing in the healthcare field due to their tunable surface properties. In this study, ceftriaxone-loaded solid lipid nanoparticles (CL-SLNPs) were synthesized using a water-in-oil-in-water (w/o/w) type double emulsification method. The formulation was optimized using response surface methodology (RSM) and was characterized using transmission electron microscopy (TEM), photon correlation spectroscopy (PCS), and UV vis and Fourier transform infrared (FTIR) spectroscopy. The CL-SLNPs were of spherical shape, 15-20 nm in size, and retained the properties of the drug or other constituents/ingredients after loading. The prepared nanoformulation offered sustained drug release after 24 h, while ceftriaxone sodium followed a burst release. Antibacterial activity of the nanoformulation was evaluated against different gram-positive and gram-negative bacterial strains. The minimum inhibitory concentration of CL-SLNPs against Pseudomonas aeruginosa was determined as 31 mu g/mL. (C) 2016 Elsevier Ireland Ltd. All rights reserved.