Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines
- Title
- Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines
- Author
- Binas, Bert
- Keywords
- Stem cells; Blastocyst; Mice; Rats; Extraembryonic endoderm
- Issue Date
- 2018-07
- Publisher
- Elsevier
- Citation
- Stem Cell Research, v. 30, Page. 100-112
- Abstract
- Mouse blastocysts contain the committed precursors of the extraembryonic endoderm (ExEn), which express the key transcription factor Oct4, depend on LIF/LIF-like factor-driven Jak/Stat signaling, and initially exhibit lineage plasticity. Previously described rat blastocyst-derived ExEn precursor-like cell lines (XENP cells/HypoSCs) also show these features, but equivalent mouse blastocyst-derived cell lines are lacking. We now present mouse blastocyst-derived cell lines, named primitive XEN (pXEN) cells, which share these and additional characteristics with the XENP cells/HypoSCs, but not with previously known mouse blastocyst-derived XEN cell lines. Otherwise, pXEN cells are highly similar to XEN cells by morphology, lineage-intrinsic differentiation potential, and multi-gene expression profile, although the pXEN cell profile correlates better with the blastocyst stage. Finally, we show that pXEN cells easily convert into XEN-like cells but not vice versa. The findings indicate that (i) pXEN cells are more representative than XEN cells of the blastocyst stage; (ii) mouse pXEN, rather than XEN, cells are homologs of rat XENP cells/HypoSCs, which we propose to call rat pXEN cells.
- URI
- https://www.sciencedirect.com/science/article/pii/S1873506118301260https://repository.hanyang.ac.kr/handle/20.500.11754/81208
- ISSN
- 1873-5061
- DOI
- 10.1016/j.scr.2018.05.008
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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