Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Binas, Bert | - |
dc.date.accessioned | 2019-01-10T02:12:43Z | - |
dc.date.available | 2019-01-10T02:12:43Z | - |
dc.date.issued | 2018-07 | - |
dc.identifier.citation | Stem Cell Research, v. 30, Page. 100-112 | en_US |
dc.identifier.issn | 1873-5061 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1873506118301260 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/81208 | - |
dc.description.abstract | Mouse blastocysts contain the committed precursors of the extraembryonic endoderm (ExEn), which express the key transcription factor Oct4, depend on LIF/LIF-like factor-driven Jak/Stat signaling, and initially exhibit lineage plasticity. Previously described rat blastocyst-derived ExEn precursor-like cell lines (XENP cells/HypoSCs) also show these features, but equivalent mouse blastocyst-derived cell lines are lacking. We now present mouse blastocyst-derived cell lines, named primitive XEN (pXEN) cells, which share these and additional characteristics with the XENP cells/HypoSCs, but not with previously known mouse blastocyst-derived XEN cell lines. Otherwise, pXEN cells are highly similar to XEN cells by morphology, lineage-intrinsic differentiation potential, and multi-gene expression profile, although the pXEN cell profile correlates better with the blastocyst stage. Finally, we show that pXEN cells easily convert into XEN-like cells but not vice versa. The findings indicate that (i) pXEN cells are more representative than XEN cells of the blastocyst stage; (ii) mouse pXEN, rather than XEN, cells are homologs of rat XENP cells/HypoSCs, which we propose to call rat pXEN cells. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (2017R1D1A1B03030942 & 2011-0030049). We thank Dr. Jin Choul Chai (Albert Einstein College of Medicine) for advice with bioinformatics. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Stem cells | en_US |
dc.subject | Blastocyst | en_US |
dc.subject | Mice | en_US |
dc.subject | Rats | en_US |
dc.subject | Extraembryonic endoderm | en_US |
dc.title | Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines | en_US |
dc.type | Article | en_US |
dc.relation.volume | 30 | - |
dc.identifier.doi | 10.1016/j.scr.2018.05.008 | - |
dc.relation.page | 100-112 | - |
dc.relation.journal | Stem Cell Research | - |
dc.contributor.googleauthor | Zhong, Yixiang | - |
dc.contributor.googleauthor | Choi, Taewoong | - |
dc.contributor.googleauthor | Kim, Minjae | - |
dc.contributor.googleauthor | Jung, Kyoung Hwa | - |
dc.contributor.googleauthor | Chai, Young Gyu | - |
dc.contributor.googleauthor | Binas, Bert | - |
dc.relation.code | 2018041619 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MOLECULAR AND LIFE SCIENCE | - |
dc.identifier.pid | bbinas | - |
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