69 0

Cloning and expression of a prion protein (PrP) gene from Korean bovine (Bos taurus coreanae) and production of rabbit anti-bovine PrP antibody

Title
Cloning and expression of a prion protein (PrP) gene from Korean bovine (Bos taurus coreanae) and production of rabbit anti-bovine PrP antibody
Author
류종석
Keywords
anti-bovine prion protein antibody; Korean bovine; prion protein; prion protein gene
Issue Date
2008-10
Publisher
SPRINGER
Citation
BIOTECHNOLOGY LETTERS, v. 30, No. 10, Page. 1705-1711
Abstract
A PrP gene, from a Korean bovine, exhibiting a nonsense and a missense polymorphism respectively at nucleotides 576 and 652 has been cloned. The latter resulted in Glu to Lys substitution at amino acid residue 218. After expression and purification of the recombinant bovine PrP (recBoPrP) with Glu218Lys substitution, a polyclonal antibody against this protein was raised. ELISA and Western blot analysis suggested that the recBoPrP obtained in this study had a unique conformation not presented in native PrPC, and the polyclonal antibody recognized PrP in a conformation dependent manner. These reagents will be valuable tools for studying PrP conformation.
URI
https://link.springer.com/article/10.1007/s10529-008-9768-4http://repository.hanyang.ac.kr/handle/20.500.11754/80728
ISSN
0141-5492
DOI
10.1007/s10529-008-9768-4
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE