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dc.contributor.author류종석-
dc.date.accessioned2018-12-04T06:52:29Z-
dc.date.available2018-12-04T06:52:29Z-
dc.date.issued2008-10-
dc.identifier.citationBIOTECHNOLOGY LETTERS, v. 30, No. 10, Page. 1705-1711en_US
dc.identifier.issn0141-5492-
dc.identifier.urihttps://link.springer.com/article/10.1007/s10529-008-9768-4-
dc.identifier.urihttp://repository.hanyang.ac.kr/handle/20.500.11754/80728-
dc.description.abstractA PrP gene, from a Korean bovine, exhibiting a nonsense and a missense polymorphism respectively at nucleotides 576 and 652 has been cloned. The latter resulted in Glu to Lys substitution at amino acid residue 218. After expression and purification of the recombinant bovine PrP (recBoPrP) with Glu218Lys substitution, a polyclonal antibody against this protein was raised. ELISA and Western blot analysis suggested that the recBoPrP obtained in this study had a unique conformation not presented in native PrPC, and the polyclonal antibody recognized PrP in a conformation dependent manner. These reagents will be valuable tools for studying PrP conformation.en_US
dc.description.sponsorshipThe authors thank Robert Shanafelt (Georgia Southern University) and William Titlow (University of Kentucky) for assistance with proofreading and revision of the manuscript. This work was supported by 63 Research Fund, Sungkyunkwan University, 2006 (M. Kwon).en_US
dc.language.isoen_USen_US
dc.publisherSPRINGERen_US
dc.subjectanti-bovine prion protein antibodyen_US
dc.subjectKorean bovineen_US
dc.subjectprion proteinen_US
dc.subjectprion protein geneen_US
dc.titleCloning and expression of a prion protein (PrP) gene from Korean bovine (Bos taurus coreanae) and production of rabbit anti-bovine PrP antibodyen_US
dc.typeArticleen_US
dc.relation.no10-
dc.relation.volume30-
dc.identifier.doi10.1007/s10529-008-9768-4-
dc.relation.page1705-1711-
dc.relation.journalBIOTECHNOLOGY LETTERS-
dc.contributor.googleauthorShin, Wooseok-
dc.contributor.googleauthorLee, Byungwoo-
dc.contributor.googleauthorHong, Sungyoul-
dc.contributor.googleauthorRyou, Chongsuk-
dc.contributor.googleauthorKwon, Moosik-
dc.relation.code2008201360-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidcryou2-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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