Validation of Tc-99m-labeled "4+1" fatty acids for myocardial metabolism and flow imaging Part 1: myocardial extraction and biodistribution
- Title
- Validation of Tc-99m-labeled "4+1" fatty acids for myocardial metabolism and flow imaging Part 1: myocardial extraction and biodistribution
- Author
- Bert Binas
- Keywords
- Technetium fatty acids; isolated heart; H-FABP; CD36; myocardial imaging
- Issue Date
- 2009-10
- Publisher
- ELSEVIER SCIENCE INC
- Citation
- NUCLEAR MEDICINE AND BIOLOGY, v. 36, No. 7, Page. 833-843
- Abstract
- Introduction: C-13, F-18 and I-123 fatty acids (FA) are used for myocardial imaging. Recently, our group showed that [Tc-99m]-labeled "4+1" FA are extracted into the rat and guinea pig myocardium. The present study evaluates determinants of myocardial uptake and whole body biodistribution of these FA derivatives.
Methods: Studies were performed with isolated perfused hearts of Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR) with a FAT/CD36 deficiency, as well as with heart type FA binding protein knockout mice (H-FABP)(-/-) and H-FABP(+/+). Eight 4+1-Tc-99m-FA were applied for 3 min followed by 1-min washout. A mathematical model was used to analyze FA dynamics and binding to proteins. Whole-body distribution was studied in rats with and without Tween 80. In vitro fractionation studies with [Tc-99m]-FA assessed red blood cell uptake as well as association with plasma lipoproteins very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL).
Results: Myocardial extraction was 19.0-33.0% of the infused dose in isolated WKY and 15.2-26.4% in SHR hearts. However, H-FABP(-/-) showed a marked reduction of tracer extraction [2.8+/-0.6%ID (percent injected dose) vs. 17+/-2%ID P<001]. Uptake in red blood cells (<1.2% ID) and incorporation into lipoproteins were negligible. Incubation of Tc-99m-FA with albumin reduced ventricular extraction (P<.001) into the range of established iodinated FA tracers. polyoxyethylene(20) sorbitan monooleate improved the heart-to-liver ratio in the biodistribution studies.
Conclusions: Myocardial uptake of [Tc-99m]-FA 4+1 derivatives is dependent on H-FABP. These substances may therefore provide a new tool to specifically assess regional myocardial changes of H-FABP. (C) 2009 Elsevier Inc. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0969805109001607?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/76472
- ISSN
- 0969-8051
- DOI
- 10.1016/j.nucmedbio.2009.06.009
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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