Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bert Binas | - |
dc.date.accessioned | 2018-10-11T06:55:31Z | - |
dc.date.available | 2018-10-11T06:55:31Z | - |
dc.date.issued | 2009-10 | - |
dc.identifier.citation | NUCLEAR MEDICINE AND BIOLOGY, v. 36, No. 7, Page. 833-843 | en_US |
dc.identifier.issn | 0969-8051 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0969805109001607?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/76472 | - |
dc.description.abstract | Introduction: C-13, F-18 and I-123 fatty acids (FA) are used for myocardial imaging. Recently, our group showed that [Tc-99m]-labeled "4+1" FA are extracted into the rat and guinea pig myocardium. The present study evaluates determinants of myocardial uptake and whole body biodistribution of these FA derivatives. Methods: Studies were performed with isolated perfused hearts of Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR) with a FAT/CD36 deficiency, as well as with heart type FA binding protein knockout mice (H-FABP)(-/-) and H-FABP(+/+). Eight 4+1-Tc-99m-FA were applied for 3 min followed by 1-min washout. A mathematical model was used to analyze FA dynamics and binding to proteins. Whole-body distribution was studied in rats with and without Tween 80. In vitro fractionation studies with [Tc-99m]-FA assessed red blood cell uptake as well as association with plasma lipoproteins very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Results: Myocardial extraction was 19.0-33.0% of the infused dose in isolated WKY and 15.2-26.4% in SHR hearts. However, H-FABP(-/-) showed a marked reduction of tracer extraction [2.8+/-0.6%ID (percent injected dose) vs. 17+/-2%ID P<001]. Uptake in red blood cells (<1.2% ID) and incorporation into lipoproteins were negligible. Incubation of Tc-99m-FA with albumin reduced ventricular extraction (P<.001) into the range of established iodinated FA tracers. polyoxyethylene(20) sorbitan monooleate improved the heart-to-liver ratio in the biodistribution studies. Conclusions: Myocardial uptake of [Tc-99m]-FA 4+1 derivatives is dependent on H-FABP. These substances may therefore provide a new tool to specifically assess regional myocardial changes of H-FABP. (C) 2009 Elsevier Inc. All rights reserved. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE INC | en_US |
dc.subject | Technetium fatty acids | en_US |
dc.subject | isolated heart | en_US |
dc.subject | H-FABP | en_US |
dc.subject | CD36 | en_US |
dc.subject | myocardial imaging | en_US |
dc.title | Validation of Tc-99m-labeled "4+1" fatty acids for myocardial metabolism and flow imaging Part 1: myocardial extraction and biodistribution | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 36 | - |
dc.identifier.doi | 10.1016/j.nucmedbio.2009.06.009 | - |
dc.relation.page | 833-843 | - |
dc.relation.journal | NUCLEAR MEDICINE AND BIOLOGY | - |
dc.contributor.googleauthor | Mirtschink, Peter | - |
dc.contributor.googleauthor | Stehr, Sebastian N. | - |
dc.contributor.googleauthor | Walther, Martin | - |
dc.contributor.googleauthor | Pietzsch, Jens | - |
dc.contributor.googleauthor | Bergmann, Ralf | - |
dc.contributor.googleauthor | Pietzsch, Hans-Juergen | - |
dc.contributor.googleauthor | Weichsel, Johannes | - |
dc.contributor.googleauthor | Pexa, Annette | - |
dc.contributor.googleauthor | Dieterich, Peter | - |
dc.contributor.googleauthor | Binas, Bert | - |
dc.contributor.googleauthor | Wunderlich, Gerd | - |
dc.contributor.googleauthor | Kropp, Joachim | - |
dc.contributor.googleauthor | Deussen, Andreas | - |
dc.relation.code | 2009207170 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MOLECULAR AND LIFE SCIENCE | - |
dc.identifier.pid | bbinas | - |
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