Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy
- Title
- Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy
- Author
- 김종호
- Keywords
- Silver nanoparticles; Optically active materials; Magnetic materials; Polymeric materials; Surface-enhanced Raman scattering
- Issue Date
- 2009-08
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Citation
- ANALYTICAL BIOCHEMISTRY, v. 391, No. 1, Page. 24-30
- Abstract
- This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8 mu m diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as Substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology. (C) 2009 Elsevier Inc. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0003269709003236?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/76270
- ISSN
- 0003-2697
- DOI
- 10.1016/j.ab.2009.05.005
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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