Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells

Title
Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
Author
노재숙
Keywords
Lactobacillus; vaginal epithelial cell; cell viability; cell proliferation
Issue Date
2011-09
Publisher
KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI, DEPT PARASITOLOGY, SEOUL, 00000, SOUTH KOREA
Citation
The Korean journal of parasitology, Vol.49, No.3, p295-298
Abstract
Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells.
URI
http://parasitol.kr/journal/view.php?doi=10.3347/kjp.2011.49.3.295http://repository.hanyang.ac.kr/handle/20.500.11754/74719
ISSN
0023-4001
DOI
10.3347/kjp.2011.49.3.295
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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