Eckol suppresses maintenance of sternness and malignancies in glioma stem-like cells
- Title
- Eckol suppresses maintenance of sternness and malignancies in glioma stem-like cells
- Author
- 이수재
- Keywords
- Eckol; Glioma stem-like cells; Self-renewal; Malignant phenotype; Resistance to anticancer treatments
- Issue Date
- 2011-06
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
- Citation
- Toxicology and Applied Pharmacology, VOL,254, NO.1, JUL 1 2011 , pp.32-40
- Abstract
- A subpopulation of cancer cells with stem cell properties is responsible for tumor maintenance and progression, and may contribute to resistance to anticancer treatments. Thus, compounds that target cancer stem-like cells could be usefully applied to destroy cancer. In this study, we investigated the effect of Eckol, a phlorotannin compound, on sternness and malignancies in glioma stem-like cells. To determine whether Eckol targets glioma stem-like cells, we examined whether Eckol treatment could change the expression levels of glioma stem-like cell markers and self-renewal-related proteins as well as the sphere forming ability, and the sensitivity to anticancer treatments. Alterations in the malignant properties of sphere-derived cells by Eckol were also investigated by soft-agar colony forming assay, by xenograft assay in nude mice, and by cell invasion assay.Treatment of sphere-forming glioma cells with Eckol effectively decreased the sphere formation as well as the CD133(+) cell population. Eckol treatment suppressed expression of the glioma stem-like cell markers and the self-renewal-related proteins without cell death. Moreover, treatment of glioma stem-like cells with Eckol significantly attenuated anchorage-independent growth on soft agar and tumor formation in xenograft mice. Importantly, Eckol treatment effectively reduced the resistance of glioma stem-like cells to ionizing radiation and temozolomide. Treatment of glioma stem-like cells with Eckol markedly blocked both phosphoinositide 3-kinase-Akt and Ras-Raf-1-Erk signaling pathways.These results indicate that the natural phlorotannin Eckol suppresses sternness and malignancies in glioma stem-like cells, and thereby makes glioma stem-like cells more sensitive to anticancer treatments, providing novel therapeutic strategies targeting specifically cancer stem-like cells.
- URI
- https://www.sciencedirect.com/science/article/pii/S0041008X11001359?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/73571
- ISSN
- 0041-008X
- DOI
- 10.1016/j.taap.2011.04.006
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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