Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김희진 | - |
dc.date.accessioned | 2018-07-27T05:35:30Z | - |
dc.date.available | 2018-07-27T05:35:30Z | - |
dc.date.issued | 2011-09 | - |
dc.identifier.citation | NEUROSCIENCE LETTERS, Vol.504, No.2 [2011], p107-111 | en_US |
dc.identifier.issn | 0304-3940 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/abs/pii/S0304394011012730 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/73548 | - |
dc.description.abstract | Human erythropoietin (hEPO) has multiple actions in non-hematopoietic tissues, including neurotrophic, anti-oxidant, anti-apoptotic, and anti-inflammatory effects. To examine the effect of EPO in an vitro model of amyotrophic lateral sclerosis (ALS), we stably overexpressed wild SOD1 and a mutant form. SOD1/G93A, in NSC-34 motoneuron-like cells. Transformants harboring the wild and mutant forms of SOD1 were selected by G418 selection and immunoblot analysis. RT-PCR analysis showed that cox-2 expression was increased in the NSC-34/mSOD1s, and MU assays and BrdU-ELISAs revealed reduced cell growth and proliferation in the NSC-34/mSOD1 cell line. Incubation with 5 or 10 IU/mL rhEPO increased the viability and decreased the cox-2 expression in the dNSC-34/mSOD1s cells. Immunocytochemical staining with anti-SOD1 antibody revealed the presence of aggregates of mSOD1 protein in dNSC-34/mSOD1 cells. Incubation with10 IU/mL rhEPO reduced the proportion of cells containing such aggregates. Our findings suggest that the anti-oxidant and anti-inflammatory effects of EPO increase the survival of NSC-34/mSOD1 cells and reduce aggregation of the mutant SOD1 protein. | en_US |
dc.description.sponsorship | This work was supported by a grant from Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2008-313-E00480). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER IRELAND LTD, ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND | en_US |
dc.subject | Erythropoietin | en_US |
dc.subject | ALS | en_US |
dc.subject | SOD1 | en_US |
dc.subject | Aggregation | en_US |
dc.title | Recombinant human erythropoietin reduces aggregation of mutant Cu/Zn-binding superoxide dismutase (SOD1) in NSC-34 cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 504 | - |
dc.identifier.doi | 10.1016/j.neulet.2011.09.008 | - |
dc.relation.page | 107-111 | - |
dc.relation.journal | NEUROSCIENCE LETTERS | - |
dc.contributor.googleauthor | Cho, Goang-Won | - |
dc.contributor.googleauthor | Kim, Ga-Young | - |
dc.contributor.googleauthor | Baek, Soojeong | - |
dc.contributor.googleauthor | Kim, Heejaung | - |
dc.contributor.googleauthor | Kim, Taikon | - |
dc.contributor.googleauthor | Kim, Hee Jin | - |
dc.contributor.googleauthor | Kim, Seung Hyun | - |
dc.relation.code | 2011207047 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hyumcbrain | - |
dc.identifier.researcherID | 56982299900 | - |
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