Sequence analysis of genomic DNA (680 Mb) by GS-FLX-Titanium sequencer in the monogonont rotifer, Brachionus ibericus
- Title
- Sequence analysis of genomic DNA (680 Mb) by GS-FLX-Titanium sequencer in the monogonont rotifer, Brachionus ibericus
- Author
- 이재성
- Keywords
- Mongonont rotifer; Brachionus ibericus; GS-FLX-Titanium; Genomic DNA; Unigene
- Issue Date
- 2011-02
- Publisher
- Springer
- Citation
- Hydrobiologia, 2011, 662, P.65-75
- Abstract
- The monogonont rotifer, Brachionus ibericus (S type), is considered to be a promising model species for developmental biology, evolution, and environmental genomics. In an attempt to accelerate the molecular understanding of B. ibericus, we sequenced 680.5 Mb of genomic DNA using the genome sequencer GS-FLX-Titanium. We obtained 2,062,621 reads (average read length 329.9 bp) and 145,418 contigs (total contigs length 125.7 Mb) after excluding small reads (less than 200 bp) from the assembly, and finally obtained 10,133 unigenes (E value a parts per thousand currency sign 9.00E-04) after non-redundant (NR) BLAST search. In this article, we summarize the genomic DNA sequences of B. ibericus and discuss their potential use in the study of reproductive biology, endocrinology, environmental genomics, and ecotoxicological studies, and for providing insight into the genetic basis of mechanisms such as egg formation, antioxidant stress defense, and xenobiotic metabolism.
- URI
- http://link.springer.com/article/10.1007/s10750-010-0516-0https://repository.hanyang.ac.kr/handle/20.500.11754/72703
- ISSN
- 0018-8158; 1573-5117
- DOI
- 10.1007/s10750-010-0516-0
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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