Signalling pathways associated with IL-6 production and epithelial-mesenchymal transition induction in prostate epithelial cells stimulated with Trichomonas vaginalis

Title
Signalling pathways associated with IL-6 production and epithelial-mesenchymal transition induction in prostate epithelial cells stimulated with Trichomonas vaginalis
Author
류재숙
Keywords
epithelial-mesenchymal transition; inflammation; interleukin-6; prostate epithelial cell; signalling pathway; Trichomonas vaginalis
Issue Date
2016-06
Publisher
WILEY-BLACKWELL
Citation
PARASITE IMMUNOLOGY, v. 38, NO 11, Page. 678-687
Abstract
Trichomonas vaginalis (Tv) has been found in patient tissue of benign prostatic hyperplasia (BPH), and suggested to cause chronic prostatitis. IL-6 is known as one of the important factors of chronic inflammation in prostate cancer. Patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) had higher levels of IL-6 in seminal plasma. Furthermore, inflammatory conditions induced by pathogen infections have been shown to promote epithelial-mesenchymal transition (EMT). Here, we investigated the signals involved in IL-6 production by human prostate epithelial cells (PECs) stimulated with Tv and examined whether Tv induces EMT in PECs. We found that PECs stimulated with Tv increased the production of IL-6, as well as the expression of TLR2, TLR4, MAPKs (p38, JNK, ERK), NF-kappa B and JAK2/STAT3, and levels of ROS. Inhibition of TLR2 or TLR4 reduced IL-6 production as well as expression of these other factors, and agents inhibiting ROS, MAPKs, NF-kappa B and JAK reduced IL-6 production. However, when PECs were stimulated with Tv, transcripts of mesenchymal cell markers increased, and epithelial cell markers decreased. In addition, the induction of EMT was suppressed by inhibitors of JAK or NF-kappa B. These findings are the first evidence that Tv infection of prostate epithelial cells may induce EMT.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1111/pim.12357http://repository.hanyang.ac.kr/handle/20.500.11754/71650
ISSN
0141-9838; 1365-3024
DOI
10.1111/pim.12357
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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