Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김계성 | - |
dc.date.accessioned | 2018-04-19T11:34:58Z | - |
dc.date.available | 2018-04-19T11:34:58Z | - |
dc.date.issued | 2013-01 | - |
dc.identifier.citation | BioMed Research International , 2013, 2013 | en_US |
dc.identifier.issn | 2314-6133 | - |
dc.identifier.uri | https://www.hindawi.com/journals/bmri/2013/143028/ | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/69789 | - |
dc.description.abstract | Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from the lack of the direct induction of ESC-like cells from well-characterized SSCs. Thus, the aim of the present study was to find and characterize pluripotent human SSCs in in vitro cultures of characterized SSCs. Human testicular tissues were dissociated and plated onto gelatin/laminin-coated dishes to isolate SSCs. In the presence of growth factors SSCs formed multicellular clumps after 2-4 weeks of culture. At passages 1 and 5, the clumps were dissociated and were then analyzed using markers of pluripotent cells. The number of SSEA-4-positive cells was extremely low but increased gradually up to similar to 10% in the SSC clumps during culture. Most of the SSEA-4-negative cells expressed markers for SSCs, and some cells coexpressed markers of both pluripotent and germ cells. The pluripotent cells formed embryoid bodies and teratomas that contained derivatives of the three germ layers in SCID mice. These results suggest that the pluripotent cells present within the clumps were derived directly from SSCs during in vitro culture. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Hindawi Publishing Corporation | en_US |
dc.subject | LONG-TERM PROLIFERATION | en_US |
dc.subject | ADULT HUMAN TESTIS | en_US |
dc.subject | MOUSE TESTIS | en_US |
dc.subject | GENERATION | en_US |
dc.subject | GROWTH | en_US |
dc.subject | PROGENITORS | en_US |
dc.subject | DERIVATION | en_US |
dc.subject | RAT | en_US |
dc.title | In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1155/2013/143028 | - |
dc.relation.journal | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | - |
dc.contributor.googleauthor | Jung Jin, Lim | - |
dc.contributor.googleauthor | Hyung Joon, Kim | - |
dc.contributor.googleauthor | Kye-Seong, Kim | - |
dc.contributor.googleauthor | Jae Yup, Hong | - |
dc.contributor.googleauthor | Dong Ryul, Lee | - |
dc.relation.code | 2011213367 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | ks66kim | - |
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